Cloud-clone
SKU:LAB080Hu71
Biotin-Linked Polyclonal Antibody to S100 Calcium Binding Protein A12 (S100A12)
Biotin-Linked Polyclonal Antibody to S100 Calcium Binding Protein A12 (S100A12)
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;Immunocytochemistry in formalin fixed cells: 5-20ug/ml
;Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
;Immunohistochemistry in paraffin section: 5-20ug/ml
;Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
;Optimal working dilutions must be determined by end user.
Product No.
LAB080Hu71
Organism Species
Homo sapiens (Human).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
PAB080Hu01-Polyclonal Antibody to S100 Calcium Binding Protein A12 (S100A12)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against S100A12. It has been selected for its ability to recognize S100A12 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Inflammation Research | Correlation of human S100A12 (EN-RAGE) and high-sensitivity C-reactive protein as gingival crevicular fluid and serum markers of inflammation in chronic periodontitis and type 2 diabetes Pubmed: 24378957 |
| Dig Dis Sci | The Circulating Level of Soluble Receptor for Advanced Glycation End Products Displays Different Patterns in Ulcerative Colitis and Crohn’s Disease: A Cross-Sectional Study PubMed: 25757448 |
| The Journal of Clinical Endocrinology & Metabolism | Low Endogenous Secretory Receptor for Advanced Glycation End-Products Levels Are Associated With Inflammation and Carotid Atherosclerosis in Prediabetes Pubmed:26885882 |
| Nutrition, Metabolism, and Cardiovascular Diseases | High intake of dietary advanced glycation end-products is associated with increased arterial stiffness and inflammation in subjects with type 2 diabetes. pubmed:28958695 |
| The Journal of Clinical Endocrinology & Metabolism | HbA1c Identifies Subjects With Prediabetes and Subclinical Left Ventricular Diastolic Dysfunction. pubmed:28973588 |
| Journal of Immunology Research | A Granulocyte-Specific Protein S100A12 as a Potential Prognostic Factor Affecting Aggressiveness of Therapy in Patients with Juvenile Idiopathic Arthritis Doi: 10.1155/2018/5349837 |
| Al-Azhar Assiut Medical Journal | The role of serum S100A12 (calgranulin C) as a diagnostic marker in Egyptian patients with irritable bowel syndrome and ulcerative colitis Doi: 10.4103/AZMJ.AZMJ_52_18 |
| Acta Diabetologica | Analysis of S100A12 plasma levels in hyperlipidemic subjects with or without familial hypercholesterolemia Pubmed: 30963307 |
| Cells | 1 h Postload Glycemia Is Associated with Low Endogenous Secretory Receptor for Advanced Glycation End Product Levels and Early Markers of Cardiovascular … |
| Biomolecules . | Multimarker Approach to Identify Patients with Coronary Artery Disease at High Risk for Subsequent Cardiac Adverse Events: The Multi-Biomarker Study Pubmed: 32549327 |
| Mediators of Inflammation | Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris |
| Animals (Basel) | The Serum and Saliva Proteome of Dogs with Diabetes Mellitus 33271797 |
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