Cloud-clone
SKU:LAC921Hu71
Biotin-Linked Polyclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB)
Biotin-Linked Polyclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB)
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Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAC921Hu71
Organism Species
Homo sapiens (Human).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
Mus musculus (Mouse), Rattus norvegicus (Rat)
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against PDGFB. It has been selected for its ability to recognize PDGFB in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| AAPS PharmSciTech | Inhibition of Glomerular Mesangial Cell Proliferation by siPDGF-B-and siPDGFR-β-Containing Chitosan Nanoplexes pubmed:27975193 |
| Marmara Pharmaceutical Journal | In vitro PDGF-B gene silencing studies and In vivo delivery of siRNA to the rat kidney using Chitosan/siRNA nanoplexes 5000184338 |
| International Journal of Clinical and Experimental Pathology | Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens ISSN:1936-2625/IJCEP0058424 |
| Cell Biology International | Melatonin promotes osteoblastic differentiation and regulates PDGF/AKT signaling pathway Pubmed: 31535749 |
| Ann Agric Environ Med | PDGF-BB homodimer serum level–a good indicator of the severity of alcoholic liver cirrhosis Pubmed: 32208584 |
| Journal of Tissue Engineering | Therapeutic potential of adipose-derived mesenchymal stem cell exosomes in tissue-engineered bladders 33868627 |
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