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SKU:LAA710Hu71

Biotin-Linked Polyclonal Antibody to Prothrombin Fragment 1+2 (F1+2)

Biotin-Linked Polyclonal Antibody to Prothrombin Fragment 1+2 (F1+2)

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UOM
Concentration
Organism species
Western blotting: 0.5-2ug/ml
;Immunocytochemistry in formalin fixed cells: 5-20ug/ml
;Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
;Immunohistochemistry in paraffin section: 5-20ug/ml
;Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
;Optimal working dilutions must be determined by end user.

Product No.

LAA710Hu71

Organism Species

Homo sapiens (Human).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

Biotin

Original Antibody

PAA710Hu01-Polyclonal Antibody to Prothrombin Fragment 1+2 (F1+2)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against F1+2. It has been selected for its ability to recognize F1+2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Critical Care Disseminated intravascular coagulation or acute coagulopathy of trauma shock early after trauma? An observational study BioMed: cc10553
Journal of Vascular Surgery Changes in thrombin generation, fibrinolysis, platelet and endothelial cell activity, and inflammation following endovascular abdominal aortic aneurysm repair ScienceDirect: S0741521411018568
Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine High levels of soluble VEGF receptor 1 early after trauma are associated with shock, sympathoadrenal activation, glycocalyx degradation and inflammation in severely injured patients: a prospective study Sjtrem:1757-7241
Journal of Thrombosis and Haemostasis High sCD40L levels early after trauma are associated with enhanced shock, sympathoadrenal activation, tissue and endothelial damage, coagulopathy and mortality Wiley: source
British Journal of Dermatology Evaluation of autologous plasma skin test in patients with chronic idiopathic urticaria PubMed: 21910697
Arteriosclerosis, Thrombosis, and Vascular Biology. Diet Modulates Endogenous Thrombin Generation, A Biological Estimate of Thrombosis Risk, Independently of the Metabolic Status PubMed: 22859493
Journal of Vascular Surgery Medium-term effect of endovascular and open abdominal aortic aneurysm repair on thrombin generation and fibrinolysis PubMed: 23140799
PLoS ONE Markers of Thrombogenesis and Fibrinolysis and Their Relation to Inflammation and Endothelial Activation in Patients with Idiopathic Pulmonary Arterial Hypertension Plosone: Source
Journal of Vascular Surgery Effect of endovascular and open abdominal aortic aneurysm repair on thrombin generation and fibrinolysis Pubmed: 23140799
Haemetology Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central India Pubmed: 23992124
Cogent Medicine Study the association of adiponectin with inflammation and hypercoagulability in case of type 2 diabetic subjects with renal dysfunction Tandfonline:Source
Interact Cardiovasc Thorac Surg. An ex vivo evaluation of blood coagulation and thromboresistance of two extracorporeal circuit coatings with reduced and full heparin dose Pubmed:24632424
Perfusion Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testing PubMed: 26243277
J Thromb Haemost Comparison of the Effect of Dabigatran and Dalteparin on Thrombus Stability in a Murine Model of Venous Thromboembolism PubMed: 26514101
Acta Oncologica Turcica Thrombin activatable fibrinolysis inhibitor (TAFI), tissue factor pathway inhibitor (TFPI), and prothrombin fragment 1+2 levels in patients with advanced colorectal cancer aot:AOT_49_1_6_12.pdf
Advances in Medical Sciences Perioperative thrombocytopenia predicts poor outcome in patients undergoing transcatheter aortic valve implantation pubmed:29145170
Thrombosis Research The causes of thrombocytopenia after transcatheter aortic valve implantation pubmed:28582640
Acta Haematologica Elevations of Thrombotic Biomarkers in Hemoglobin H Disease Pubmed:29402840
Journal of Neurotrauma The cerebral thrombin system is activated after intracerebral hemorrhage and contributes to secondary lesion growth and poor neurological outcome in C57Bl/6 mice Pubmed: 31830857
Indian Journal of Public Health Research & Development Enhanced Fibrin-Lysis in Grade-1 Dengue Haemorrhagic Fever
Rheumatol Int The impact of anti-endothelial cell antibodies (AECAs) on the development of blood vessel damage in patients with systemic lupus erythematosus: the preliminary … Pubmed:35284968
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