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SKU:LAC228Mu71
Biotin-Linked Polyclonal Antibody to Occludin (OCLN)
Biotin-Linked Polyclonal Antibody to Occludin (OCLN)
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Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAC228Mu71
Organism Species
Mus musculus (Mouse).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against OCLN. It has been selected for its ability to recognize OCLN in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Brain Research | HIV-1 Tat protein increases the permeability of brain endothelial cells by both inhibiting occludin expression and cleaving occludin via matrix metalloproteinase-9 ScienceDirect: S0006899311021408 |
| Plos one | Disruption of the Circadian Clock in Mice Increases Intestinal Permeability and Promotes Alcohol-Induced Hepatic Pathology and Inflammation PubMed: PMC3688973 |
| Journal of Neurotrauma | A new panel of blood biomarkers for the diagnosis of mild traumatic brain injury/concussion in adults PubMed: 25794137 |
| United States Patent Application | Methods for Diagnosis and Treatment of Concussion or Brain Injury 2016:0103140 |
| Sci Rep. | Blood Occludin Level as a Potential Biomarker for Early Blood Brain Barrier Damage Following Ischemic Stroke articles:srep40331 |
| Frontiers in Pharmacology | Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal … Pubmed:29375374 |
| Thorax | Fas activation alters tight junction proteins in acute lung injury Pubmed: 30385692 |
| Antioxidants (Basel). | The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned Piglets Pubmed: 31426309 |
| DISEASE MARKERS | Peripheral blood occludin level as a biomarker for preoperative cerebral edema in patients with brain tumors Pubmed: 32884584 |
| Burns Open | Intestinal Permeability in Participants With Thermal Injury: A Case Series From a Prospective, Longitudinal Study (HESTIA) |
| PLoS One | A comparison of the effects of monotherapy with rosuvastatin, atorvastatin or ezetimibe versus combination treatment with rosuvastatin-ezetimibe and … 34492054 |
| CNS Neuroscience & Therapeutics | Higher serum occludin after successful reperfusion Is associated with early neurological deterioration Pubmed:35338575 |
| Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation … |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC228Mu01 | Recombinant Occludin (OCLN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC228Mu03 | Recombinant Occludin (OCLN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC228Mu02 | Recombinant Occludin (OCLN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC228Mu04 | Recombinant Occludin (OCLN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC228Mu05 | Recombinant Occludin (OCLN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC228Mu02 | Polyclonal Antibody to Occludin (OCLN) | WB; IHC; ICC; IP. |
| PAC228Mu01 | Polyclonal Antibody to Occludin (OCLN) | WB; IHC; ICC; IP. |
| LAC228Mu71 | Biotin-Linked Polyclonal Antibody to Occludin (OCLN) | WB; IHC; ICC. |
| SEC228Mu | ELISA Kit for Occludin (OCLN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC228Mu | Magnetic Luminex Assay Kit for Occludin (OCLN) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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