Cloud-clone
SKU:LAC284Mu71
Biotin-Linked Polyclonal Antibody to Afamin (AFM)
Biotin-Linked Polyclonal Antibody to Afamin (AFM)
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Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAC284Mu71
Organism Species
Mus musculus (Mouse).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against AFM. It has been selected for its ability to recognize AFM in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Journal of Proteomics | 2D-DIGE analysis of sera from transgenic mouse models reveals novel candidate protein biomarkers for human gastric cancer ScienceDirect: S1874391912004964 |
| Clin Chim Acta | Afamin — A pleiotropic glycoprotein involved in various disease states PubMed: 25892677 |
| Nutrients | Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after … Pubmed:30037068 |
| Biomarkers in Medicine | Urine afamin and afamin–creatinine ratio as biomarkers for kidney injury Pubmed: 30433819 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC284Mu01 | Recombinant Afamin (AFM) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC284Mu01 | Polyclonal Antibody to Afamin (AFM) | WB; IHC; ICC; IP. |
| LAC284Mu81 | FITC-Linked Polyclonal Antibody to Afamin (AFM) | WB; IHC; ICC; IF. |
| LAC284Mu71 | Biotin-Linked Polyclonal Antibody to Afamin (AFM) | WB; IHC; ICC. |
| KSC284Mu01 | ELISA Kit DIY Materials for Afamin (AFM) | Main materials for "Do It (ELISA Kit) Yourself". |
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