Cloud-clone
SKU:LAC343Mu71
Biotin-Linked Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)
Biotin-Linked Polyclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)
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Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAC343Mu71
Organism Species
Mus musculus (Mouse).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against HSPA5. It has been selected for its ability to recognize HSPA5 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Heliyon | Glucose-regulated protein 78 (GRP78) in renal cell carcinoma: A novel biomarker for predicting tumor behavior |
| Medicine (Baltimore) | Levels of circulating GRP78 and CHOP in endoplasmic reticulum stress pathways in Chinese type 2 diabetic kidney disease patients 34414940 |
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