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SKU:LAB250Mu71

Biotin-Linked Polyclonal Antibody to Cluster Of Differentiation 73 (CD73)

Biotin-Linked Polyclonal Antibody to Cluster Of Differentiation 73 (CD73)

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UOM
Concentration
Organism species
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Product No.

LAB250Mu71

Organism Species

Mus musculus (Mouse).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

Biotin

Original Antibody

PAB250Mu01-Polyclonal Antibody to Cluster Of Differentiation 73 (CD73)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CD73. It has been selected for its ability to recognize CD73 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Journal of Autoimmunity The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation Pubmed:25640206
Talanta Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment … 10.1016:j.talanta.2018.01.044
Cancer Biomarkers Expression and clinical significance of serum NT5E protein in patients with colorectal cancer Pubmed: 30932882
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB250Mu01 Recombinant Cluster Of Differentiation 73 (CD73) Positive Control; Immunogen; SDS-PAGE; WB.
PAB250Mu01 Polyclonal Antibody to Cluster Of Differentiation 73 (CD73) WB; IHC; ICC; IP.
LAB250Mu81 FITC-Linked Polyclonal Antibody to Cluster Of Differentiation 73 (CD73) WB; IHC; ICC; IF.
LAB250Mu71 Biotin-Linked Polyclonal Antibody to Cluster Of Differentiation 73 (CD73) WB; IHC; ICC.
KSB250Mu01 ELISA Kit DIY Materials for Cluster Of Differentiation 73 (CD73) Main materials for "Do It(ELISA Kit) Yourself"

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