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SKU:LAB447Ra71

Biotin-Linked Polyclonal Antibody to Acetylcholinesterase (ACHE)

Biotin-Linked Polyclonal Antibody to Acetylcholinesterase (ACHE)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

LAB447Ra71

Organism Species

Rattus norvegicus (Rat).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

Biotin

Original Antibody

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ACHE. It has been selected for its ability to recognize ACHE in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Acta Histochem Cytochem.

The Effects of Exercise-induced Fatigue on Acetylcholinesterase Expression and Activity at Rat Neuromuscular Junctions PubMed: PMC2775104

Applied Biological Chemistry

Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation 10.1007/s13765-017-0327-3

Molecular Nutrition & Food Research

Thymoquinone Can Improve Neuronal Survival and Promote Neurogenesis in Rat Hippocampal Neurons Pubmed:29277983

Inflammation

Feishu Acupuncture Inhibits Acetylcholine Synthesis and Restores Muscarinic Acetylcholine Receptor M2 Expression in the Lung When Treating Allergic Asthma Pubmed:29520557

International Journal of Molecular Sciences

In Vitro Evaluation and Docking Studies of 5-oxo-5H-furo [3, 2-g] chromene-6-carbaldehyde Derivatives as Potential Anti-Alzheimer's Agents Pubmed: 31683761

BMC Anesthesiology

Cerebrospinal fluid cholinergic biomarkers are associated with postoperative delirium in elderly patients undergoing Total hip/knee replacement: a … Pubmed: 32988385

PHYTOMEDICINE

Thymoquinone administration ameliorates Alzheimer's disease-like phenotype by promoting cell survival in the hippocampus of amyloid beta1-42 infused rat … Pubmed: 32920292

PLoS One

Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats Pubmed:35421180

scientific reports

Neuropsychopharmacological profiling of scoparone in mice Pubmed:35039558

SAUDI J BIOL SCI

Aqueous Ajwa dates seeds extract improves memory impairment in type-2 diabetes mellitus rats by reducing blood glucose levels and enhancing brain … Pubmed:35531250

Saudi Pharmaceutical Journal

Sukkari dates seed improves type-2 diabetes mellitus-induced memory impairment by reducing blood glucose levels and enhancing brain cholinergic … Pubmed:35812141


Catalog No.

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