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SKU:LAB477Ra71

Biotin-Linked Polyclonal Antibody to Matrix Gla Protein (MGP)

Biotin-Linked Polyclonal Antibody to Matrix Gla Protein (MGP)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

LAB477Ra71

Organism Species

Rattus norvegicus (Rat).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

Biotin

Original Antibody

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against MGP. It has been selected for its ability to recognize MGP in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Nephrology Dialysis Transplantation

Magnesium prevents phosphate-induced calcification in human aortic vascular smooth muscle cells PubMed: PMC3611891

Maturitas.

Bone metabolism regulators and arterial stiffness in postmenopausal women Pubmed: 23916080

The Egyptian Rheumatologist

Osteoprotegerin (OPG) and Matrix Gla protein (MGP) in rheumatoid arthritis patients: Relation to disease activity ScienceDirect: S1110116414000076

Toxicology Reports

Canola and hydrogenated soybean oils accelerate ectopic bone formation induced by implantation of bone morphogenetic protein in mice Researchgate:Source

Lipids in Health and Disease

Markers of increased cardiovascular risk in patients with chronic kidney disease Biomedcentral:Source

Human & Veterinary Medicine

The behavior of circulating matrix Gla protein, matrix metalloproteinase-9 and nitrotyrosine in patients with varicose veins Hvm:Source

Pol Arch Med Wewn

Effect of Vitamin K2 on progression of atherosclerosis and vascular calcification in nondialyzed patients with chronic kidney disease stages 3-5 PubMed: 26176325

Lipids Health Dis.

Markers of increased atherosclerotic risk in patients with chronic kidney disease: a preliminary study pmc:PMC4739105

Kidney Blood Press Res.

Plasma Desphospho-Uncarboxylated Matrix Gla Protein as a Marker of Kidney Damage and Cardiovascular Risk in Advanced Stage of Chronic Kidney Disease Pubmed:27100101

Archives of Oral Biology

Matrix-Gla Protein rs4236 [A/G] gene polymorphism and serum and GCF levels of MGP in patients with subgingival dental calculus pubmed:27348051

Cell Death & Disease

Mesenchymal stromal cells-derived matrix Gla protein contribute to the alleviation of experimental colitis Pubmed:29880866

Urology

High concentration of calcium promotes mineralization in NRK52E cells via inhibiting the expression of matrix Gla protein Pubmed:29935264

Clinica Chimica Acta

Serum total matrix Gla protein: Reference interval in healthy adults and variations in patients with vascular and osteoarticular diseases Pubmed: 30597137

Biological Trace Element Research

Altered Mineral Metabolism and Disequilibrium Between Calcification Promoters and Inhibitors in Chronic Hemodialysis Patients Pubmed: 30847765

Renal Failure

Relationship of matrix Gla protein and vitamin K with vascular calcification in hemodialysis patients Pubmed: 31538831

Heliyon

Matrix Gla protein maintains normal and malignant hematopoietic progenitor cells by interacting with bone morphogenetic protein-4 Pubmed: 32322728


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