Cloud-clone
SKU:LAB908Ra71
Biotin-Linked Polyclonal Antibody to Aggrecan (AGC)
Biotin-Linked Polyclonal Antibody to Aggrecan (AGC)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAB908Ra71
Organism Species
Rattus norvegicus (Rat).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against AGC. It has been selected for its ability to recognize AGC in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Experimental Biology and Medicine |
Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells Rsmjournals: Source |
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Age( dordr). |
In vitro tenocyte metabolism in aging and oestrogen deficiency Springer: Source |
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PLoS ONE |
A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source |
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Stem Cell Research & Therapy |
Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly. Pubmed: 24739658 |
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Materials Science and Engineering: C |
Co-electrospun gelatin-poly(l-lactic acid) scaffolds: Modulation of mechanical properties and chondrocyte response as a function of composition ScienceDirect: S0928493113006656 |
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PLoS One. |
Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592 |
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Connective Tissue Research |
Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes Pubmed:25333747 |
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Macromolecular Bioscience |
Highly Porous Gelatin Reinforced 4D Scaffolds for Articular Cartilage Regeneration PubMed: 25787871 |
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Osteoarthritis and Cartilage |
Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture in meniscectomized Cavia (Guinea pig )inea pigs Pubmed:26802547 |
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Cell Proliferation |
Effects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytes Pubmed:27090063 |
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bmc musculoskeletal disorders |
Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis. pubmed:28187731 |
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Journal of Clinical Medicine |
Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors Pubmed: 32392807 |
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Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs |
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researchsquare |
Aggrecan-a new biomarker for acute type A aortic dissection 33990642 |
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Catalog No. |
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