Biotin-Linked Polyclonal Antibody to Cluster Of Differentiation 73 (CD73)

Western blotting: 0.5-2ug/ml
;Immunocytochemistry in formalin fixed cells: 5-20ug/ml
;Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
;Immunohistochemistry in paraffin section: 5-20ug/ml
;Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
;Optimal working dilutions must be determined by end user.

Product No.

LAB250Ra71

Organism Species

Rattus norvegicus (Rat).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

Biotin

Original Antibody

PAB250Ra01-Polyclonal Antibody to Cluster Of Differentiation 73 (CD73)

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

Homo sapiens (Human), Mus musculus (Mouse)

Applications

WB; IHC; ICC.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CD73. It has been selected for its ability to recognize CD73 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.