Cloud-clone
SKU:LAB373Po71
Biotin-Linked Polyclonal Antibody to Defensin Beta 1 (DEFb1)
Biotin-Linked Polyclonal Antibody to Defensin Beta 1 (DEFb1)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAB373Po71
Organism Species
Sus scrofa; Porcine (Pig).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against DEFb1. It has been selected for its ability to recognize DEFb1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Appl Environ Microbiol. |
Modulation of Porcine β-Defensins 1 and 2 upon Individual and Combined Fusarium Toxin Exposure in a Swine Jejunal Epithelial Cell Line Asm: Source |
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British Journal of Nutrition |
Identification of sociodemographic and clinical factors associated with the levels of human β-defensin-1 and human β-defensin-2 in the human milk of Han Chinese Pubmed: 24124699 |
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International Journal of Dairy Technology |
Concentration characteristics of bovine β-defensin 1 and 2 in fresh bovine milk and infant formula Doi: 10.1111 |
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Molecular Nutrition Food Research |
Green tea polyphenol epigallocatechin-3-gallate improves epithelial barrier function by inducing the productionof antimicrobial peptide pBD-1 and pBD-2 in monolayers of porcine intestinal epithelial IPEC-J2 cells. Pubmed:26991948 |
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Frontiers in Microbiology |
Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host Defense Pubmed:29997590 |
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International Immunopharmacology |
Caprylic acid and nonanoic acid upregulate endogenous host defense peptides to enhance intestinal epithelial immunological barrier function via histone deacetylase … Pubmed: 30342347 |
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cyberleninka |
EFFECT OF NIOSOMAL ANTIMICROBIAL PEPTIDE HBD-1 ON THE HEALING RATE OF INFECTED WOUNDS IN RATS |
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Fundamental & clinical pharmacology |
Effect of antimicrobial peptides HNP‐1 and hBD‐1 on Staphylococcus aureus strains in vitro and in vivo Pubmed: 31313350 |
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Cytokine |
Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis Pubmed: 31927460 |
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Animals |
Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport 33375218 |
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Sci Rep |
Human beta defensin levels and vaginal microbiome composition in post-menopausal women diagnosed with lichen sclerosus 34362937 |
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J Mol Histol |
Isolation, culture, and identification of ceruminous gland cells Pubmed:35113280 |
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Catalog No. |
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