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SKU:LAB353Ge81

FITC-Linked Polyclonal Antibody to Advanced Glycation End Product (AGE)

FITC-Linked Polyclonal Antibody to Advanced Glycation End Product (AGE)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

LAB353Ge81

Organism Species

Pan-species (General).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

FITC

Original Antibody

PAB353Ge01-Polyclonal Antibody to Advanced Glycation End Product (AGE)

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Traits

Liquid

Concentration

1mg/ml

Organism Species More

Pan-species (General)

Applications

IHC; ICC;ELISA

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against AGE. It has been selected for its ability to recognize AGE in immunohistochemical staining and western blotting.

USAGE

Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Human Immunology

Threshold serum concentrations of tumour necrosis factor alpha (TNFα) as a potential marker of the presence of microangiopathy in children and adolescents with type 1 diabetes mellitus (T1DM) PubMed: S0198885912005642

Experimental Dermatology

A long-standing hyperglycaemic condition impairs skin barrier by accelerating skin ageing process PubMed: 22017743

J Hum Nutr Diet.

Daily intake of vitamin D- or calcium-vitamin D-fortified Persian yogurt drink (doogh) attenuates diabetes-induced oxidative stress: evidence for antioxidative properties of vitamin D. Pubmed: 23829785

Gene

Association of RAGE gene polymorphism with circulating AGEs level and paraoxonase activity in relation to macro-vascular complications in Indian type 2 diabetes mellitus patients. Pubmed: 23721855

Gynecol Endocrinol.

Lipoprotein-associated phospholipase A2 and AGEs are associated with cardiovascular risk factors in women with history of gestational diabetes mellitus. Pubmed:24397392

Microvasc Res.

Role of advanced glycation end product (AGE)-induced receptor (RAGE) expression in diabetic vascular complications Pubmed:24984291

Free Radic Res.

Nitroxides prevent protein glycoxidation in vitro. Pubmed:25363554

Sleep and Breathing

Serum advanced glycation end products are associated with insulin resistance in male nondiabetic patients with obstructive sleep apnea Pubmed:25566941

Chem Biol Interact

Ascorbic acid and protein glycation PubMed: 26163454

Osteoarthritis Cartilage

Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazone PubMed: 26321377

journal of human hypertension

Relationship between serum levels of endogenous secretory RAGE and blood pressure in male nondiabetic patients with obstructive sleep apnea PubMed: 25994997

Redox Biol.

Glycation of bovine serum albumin by ascorbate PubMed: 26202868

J Oral Pathol Med

Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human saliva PubMed: 26608886

J Agric Food Chem

Inhibition of Advanced Glycation Endproduct Formation by Lotus Seedpod Oligomeric Procyanidins through RAGE-MAPK Signaling and NF-κB Activation in High-Fat-Diet Rats. PubMed: 26207852

Kineziologija

ADVANCED GLYCATION END PRODUCTS'RESPONSE TO RESISTANCE TRAINING IN POSTMENOPAUSAL WOMEN WITH TYPE II DIABETES 140251:

Food Funct.

Effects of edible bird's nest on hippocampal and cortical neurodegeneration in ovariectomized rats PubMed: 25920003

Molecular and Cellular Endocrinology

Beneficial effects of urine-derived stem cells on fibrosis and apoptosis of myocardial, glomerular and bladder cells Pubmed:26952874

Advances in Experimental Medicine and Biology

Effects of S-Nitroso-N-Acetyl-Penicillamine (SNAP) on Inflammation, Lung Tissue Apoptosis and iNOS Activity in a Rabbit Model of Acute Lung Injury Pubmed:27334732

Eur J Vasc Endovasc Surg

The Receptor for Advanced Glycation End Products (Rage) and Its Ligands in Plasma and InfrainCavia (Guinea pig )inal Bypass Vein Pubmed:26905625

molecular and cellular endocrinology

Berberine exerts renoprotective effects by regulating the AGEs-RAGE signaling pathway in mesangial cells during diabetic nephropathy. pubmed:28087385

cellular physiology and biochemistry

Inhibition of Methylglyoxal-Induced AGEs/RAGE Expression Contributes to Dermal Protection by N-Acetyl-L-Cysteine. pubmed:28214842

General Physiology and Biophysics

Glutathione is the main endogenous inhibitor of protein glycation. pubmed:28218612

Osteoarthritis and Cartilage

Establishment of a rabbit model to study the influence of advanced glycation end productsaccumulation on osteoarthritis and the protective effect of pioglitazone. pubmed:26321377

Scientific Reports

Advanced glycation end products promote VEGF expression and thus choroidal neovascularization via Cyr61-PI3K/AKT signaling pathway pubmed:29097668

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE

Lychee seed extract protects against neuronal injury and improves cognitive function in rats with type II diabetes mellitus with cognitive impairment pubmed:29138799

台湾农业研究

(台灣農業研究67 (4): 355-364) 香菇柄水萃物改善糖尿病降低阿茲海默氏症罹病風險初探

Biological Trace Element Research

Altered Mineral Metabolism and Disequilibrium Between Calcification Promoters and Inhibitors in Chronic Hemodialysis Patients Pubmed: 30847765

International journal of molecular sciences

Cellular Metabolomics Reveal the Mechanism Underlying the Anti-Atherosclerotic Effects of Aspirin Eugenol Ester on Vascular Endothelial Dysfunction Pubmed: 31261711

Evidence-Based Complementary and Alternative Medicine

Fushiming Capsule Attenuates Diabetic Rat Retina Damage via Antioxidation and Anti-Inflammation

Journal of Alzheimers Disease

Effect of Advanced Glycation End Products on the Progression of Alzheimer's Disease Pubmed: 31561370

Life Sciences

Protective effect of vanillin on diabetic nephropathy by decreasing advanced glycation end products in rats Pubmed: 31759039

Journal of Diabetes Research

Supplementation with Korean Red Ginseng Improves Current Perception Threshold in Korean Type 2 Diabetes Patients: A Randomized, Double-Blind, Placebo … Pubmed: 32025522

Pharmaceuticals

A New Insight into Meloxicam: Assessment of Antioxidant and Anti-Glycating Activity in In Vitro Studies Pubmed: 32927809

Mol Cell Endocrinol

Advanced glycation end products enhance macrophage polarization to the M1 phenotype via the HIF-1α/PDK4 pathway Pubmed: 32464167

archives of gerontology and geriatrics

The effect of Korean Red Ginseng on sarcopenia biomarkers in type 2 diabetes patients Pubmed: 32470863


The Role of PPARγ in Hyperglycemia-induced Deleterious Effect on Chondrocytes

Biomolecules

Young and Undamaged rMSA Improves the Healthspan and Lifespan of Mice 34439857


Dietary Advanced Glycation End Products Shift the Gut Microbiota Composition and Induce Insulin Resistance in Mice Pubmed:35210793


Catalog No.

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Applications (RESEARCH USE ONLY!)

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Enzyme-linked immunosorbent assay for Antigen Detection.

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ELISA Kit DIY Materials for Advanced Glycation End Product (AGE)

Main materials for "Do It (ELISA Kit) Yourself".

 

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