Cloud-clone
SKU:LAA808Ge81
FITC-Linked Polyclonal Antibody to 5-Hydroxytryptamine (5-HT)
FITC-Linked Polyclonal Antibody to 5-Hydroxytryptamine (5-HT)
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Western blotting: 0.5-2ug/ml
;Immunocytochemistry in formalin fixed cells: 5-20ug/ml
;Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
;Immunohistochemistry in paraffin section: 5-20ug/ml
;Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
;Optimal working dilutions must be determined by end user.
Product No.
LAA808Ge81
Organism Species
Pan-species (General).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
FITC
Original Antibody
PAA808Ge01-Polyclonal Antibody to 5-Hydroxytryptamine (5-HT)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
Pan-species (General)
Applications
WB; IHC; ICC; IF.
If the antibody is used in flow cytometry, please check FCM antibodies
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against 5-HT. It has been selected for its ability to recognize 5-HT in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Fitoterapia |
Ginsenoside rich fraction of Panax ginseng C.A. Meyer improve feeding behavior following radiation-induced pica in rats ScienceDirect: S0367326X12001153 |
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Clinical & Experimental Allergy |
Sensitization induces hypersensitivity in trigeminal nerve PubMed: 23106664 |
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International Journal of Immunopathology & Pharmacology |
Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats PubMed: 26684638 |
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Neuropsychiatr Dis Treat |
Catha edulis chewing effects on treatment of paranoid schizophrenic patients PubMed: 25926735 |
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Neuroscience Letters |
Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depression Pubmed:26773866 |
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Gene |
Unveiling the interactions among BMPR-2, ALK-1 and 5-HTT genes in the pathophysiology of HAPE Pubmed:27196063 |
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American Journal of Animal and Veterinary Sciences |
Metabolic Features of Heart Failure with Different Etiology ofsp11075 |
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Medical Science Monitor Basic Research |
Valproate Attenuates Nitroglycerin-Induced Trigeminovascular Activation by Preserving Mitochondrial Function in a Rat Model of Migraine pubmed:27618395 |
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Molecular Medicine Reports |
PI3K/AKT signaling pathway activation in a rat model of migraine. pubmed:28791398 |
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Neuropsychiatric disease and treatment |
The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic … Pubmed:29379286 |
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Evidence-Based Complementary and Alternative Medicine |
Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile Acids Pubmed:29853946 |
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Andrologia |
Changes in sexual performance and biochemical characterisation of functional neural regions: A study in serotonin transporter knockout male rats Pubmed: 31037750 |
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Neuroscience |
Serotonin-Mediated Cardiac Analgesia Via Ah-Type Baroreceptor Activation Contributes to Silent Angina and Asymptomatic Infarction Pubmed: 31153963 |
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Frontiers in Immunology |
TLR2-Melatonin Feedback Loop Regulates the Activation of NLRP3 Inflammasome in Murine Allergic Airway Inflammation Pubmed: 32117301 |
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Journal of neural transmission (Vienna, Austria : 1996) |
Neurotransmitter system aberrations in patients with drug addiction Pubmed: 32804296 |
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河北医科大学学报 |
重组人脑利钠肽治疗慢性肺心病急性加重期的临床效果及对5-HT 浓度的影响 |
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Journal of tranditional chinese medical sciences |
Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome |
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International Journal of?Nanomedicine |
Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡ 33762821 |
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CRYOBIOLOGY |
O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress 33651993 |
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Horm Mol Biol Clin Investig |
Investigation of the effect of training on serotonin, melatonin and hematologic parameters in adolescent basketball players 34090322 |
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Dev Comp Immunol |
A specific primed immune response in red palm weevil, Rhynchophorus ferrugineus, is mediated by hemocyte differentiation and phagocytosis Pubmed:35245605 |
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Genes Brain and Behavior |
Correlation between intestinal microbiotal imbalance and 5‐HT metabolism, immune inflammation in chronic unpredictable mild stress male rats Pubmed:35535862 |
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Catalog No. |
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Applications (RESEARCH USE ONLY!) |
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LAA808Ge71 |
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LAA808Ge81 |
FITC-Linked Polyclonal Antibody to 5-Hydroxytryptamine (5-HT) |
WB; IHC; ICC; IF. |
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MEA808Ge |
Enzyme-linked immunosorbent assay for Antigen Detection. |
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CEA808Ge |
Enzyme-linked immunosorbent assay for Antigen Detection. |
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IEA808Ge |
Enzyme-linked immunosorbent assay for Antigen Detection. |
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AEA808Ge |
Enzyme-linked immunosorbent assay for Antibody Detection. |
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CCA808Ge |
Chemiluminescent immunoassay for Antigen Detection. |
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LMA808Ge |
Magnetic Luminex Assay Kit for 5-Hydroxytryptamine (5-HT) ,etc. |
Magnetic Luminex Assay for Antigen Detection. |
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KSA808Ge11 |
Main materials for "Do It (ELISA Kit) Yourself". |
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