Cloud-clone
SKU:LAA461Ge81
FITC-Linked Polyclonal Antibody to Estradiol (E2)
FITC-Linked Polyclonal Antibody to Estradiol (E2)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAA461Ge81
Organism Species
Pan-species (General).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
FITC
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IF.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against E2. It has been selected for its ability to recognize E2 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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BioMed research international |
Antiosteoclastic Activity of Milk Thistle Extract after Ovariectomy to Suppress Estrogen Deficiency-Induced Osteoporosis PubMed: PMC3678416 |
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Fertility and Sterility |
Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells Pubmed: 23954356 |
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Repositório Digital |
Utilizacao de células-tronco adultas para tratamento de falência ovariana causada por quimioterapia em camundongas Lume: Source |
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Electromagnetic Biology and Medicine |
The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats Informahealthcare: Source |
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Academic Sciences |
Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female rats Ijppsjournal: Source |
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Stem Cells Int. |
Cell Therapy for Chemically Induced Ovarian Failure in Mice Pubmed:Pmc4274854 |
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Reproductive Toxicology |
Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring Pubmed:24126190 |
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Phytomedicine. |
Phloretin promotes osteoclast apoptosis in murine macrophages and inhibits estrogen deficiency-induced osteoporosis in mice Pubmed:24932975 |
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Turkish Journal of Urology |
Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy Turkishjournalofurology:Source |
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international journal of environmental research and public health |
Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy PubMed: 25996886 |
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Reprod Toxicol |
In utero PubMed: 25530038 |
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oncology research |
Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats PubMed: 26629940 |
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Environ Toxicol |
Effect on the H19 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis period PubMed: 25782373 |
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J Endocrinol |
Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarction PubMed: 25770118 |
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Reproduction |
Prenatal and lactation nicotine exposure affects Sertoli cell and gonadotropin levels in rats PubMed: 26556892 |
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Veterinary Word |
Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers Pubmed:27051212 |
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Archives of Physiology and Biochemistry |
Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder Pubmed:26934364 |
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Plos One |
Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota Pubmed:27093642 |
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Biology of Reproduction |
Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring Pubmed:26962114 |
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PPAR Research |
Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction byUpreCavia (Guinea pig )lating PPARα Pubmed:27413362 |
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Clinical and Experimental Hypertension |
Assessment of ADMA, estradiol, and progesterone in severe preeclampsia Pubmed:27152507 |
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PLoS Genet. |
A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in Dendritogenesis Pubmed:26977770 |
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Scientific Reports |
The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilization pubmed:27876896 |
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Scientific Reports |
Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373 |
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33 |
Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389 |
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African Journal of Environmental Science and Technology |
Pollution by endocrine disrupting estrogens in aquatic ecosystems in Morogoro urban and peri-urban areas in Tanzania 150659 |
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Journal of Functional Foods |
Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women S1756464617301561 |
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environmental pollution |
Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061 |
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Environmental toxicology and pharmacology |
Combined oral contraceptive and nitric oxide synthesis inhibition synergistically causes cardiac hypertrophy and exacerbates insulin resistance in female rats. pubmed:28376377 |
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PPAR Research |
Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα pubmed:27413362 |
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Toxicological Sciences |
Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384 |
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Sci Rep |
Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. pubmed:28322267 |
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Interact Cardiovasc Thorac Surg |
Acute administration of oestradiol or progesterone in a spinal cord ischaemia–reperfusion model in rats pubmed:29049608 |
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Toxicological Sciences |
Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction pubmed:28973666 |
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Reproductive Sciences |
Abnormality of Klotho Signaling Is Involved in Polycystic Ovary Syndrome pubmed:28673204 |
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Oncotarget |
Rcan2 and estradiol independently regulate body weight in female mice pubmed:28624805 |
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African Journal of Environmental Science and Technology |
Pollution by endocrine disrupting estrogens in aquatic ecosystems in Morogoro urban and peri-urban areas in Tanzania 10.5897/AJEST2016.2234 |
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Journal of Orthopaedic Research |
The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice pubmed:29058779 |
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Animal Production Science |
Can serum levels of anti-Müllerian hormone and oestradiol in juvenile gilts be used to predict future reproductive performance? 10.1071/ANv57n12Ab065 |
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Asian Journal of Biomedical and Pharmaceutical Sciences |
A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/ò-catenin Signaling Pathways in Ovariectomized Rats ISSN 2249 – 622X |
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Nanotheranostics |
The biocompatibility studies of polymer dots on pregnant mice and fetuses pubmed:29071192 |
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DARU Journal of Pharmaceutical Sciences |
Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway pubmed:29020999 |
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Reproduction. |
Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring pubmed:28687593 |
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Molecular Medicine Reports |
Molecular mechanism and functional role of macrophage colony‑stimulating factor in follicular granulosa cells pubmed:28656272 |
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Annals of Rheumatic Diseases |
FRI0390 Impaired ovarian reserve in patients affected by systemic lupus erythematosus: a case-control study Suppl_2:728.1.abstract |
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Stem Cell Research & Therapy |
The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity Pubmed:30021657 |
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Environmental Pollution |
Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625 |
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Toxicological Sciences |
Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons 10.1093:toxsci |
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Frontiers in Molecular Neuroscience |
Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355 |
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Aquatic Toxicology |
Exposure to tris (1, 3-dichloro-2-propyl) phosphate for Two generations decreases fecundity of zebrafish at environmentally relevant concentrations Pubmed:29775925 |
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Journal of Ethnopharmacology |
Effects of water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat model Pubmed:29705515 |
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Inflammation |
Differential Effects of Brain Death on Rat Microcirculation and Intestinal Inflammation: Female Male Pubmed:29737476 |
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European Journal of Neuroscience |
Postnatal separation prevents the development of prenatal stress‐induced anxiety in association with changes in oestrogen receptor and oxytocin immunoreactivity in … Pubmed:29205599 |
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Journal of Ovarian Research |
DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway Pubmed:29321035 |
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Medical Science Monitor |
Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian … Pubmed:29674607 |
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Biomedicine & Pharmacotherapy |
Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons Doi: 10.1016/j.biopha.2018.08.135 |
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Journal of Endocrinology |
Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome Pubmed: 29535146 |
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Stem Cell Research & Therapy |
Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the … Pubmed: 30606243 |
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journal of molecular neuroscience |
Possible Metabolic Alterations among Autistic Male Children: Clinical and Biochemical Approaches Pubmed: 30600432 |
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Heliyon |
Establishment and evaluation of a novel mouse model of peri/postmenopausal depression Doi: 10.1016/j.heliyon.2019.e01195 |
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Experimental and Therapeutic Medicine |
Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary |
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Evidence-Based Complementary and Alternative Medicine |
iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine |
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Biomedicine & Pharmacotherapy |
G protein-coupled estrogen receptor activation improves contractile and diastolic functions in rat renal interlobular artery to protect against renal ischemia … Pubmed: 30784936 |
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Biomedicine & Pharmacotherapy |
Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve |
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Biology of Reproduction |
G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Accelerates Restoration of Ovarian Function in Cyclophosphamide … Pubmed: 31034039 |
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Biomedicine & pharmacotherapy |
Aqueous extract of lily bulb ameliorates menopause-like behavior in ovariectomized mice with novel brain-uterus mechanisms distinct from estrogen therapy Pubmed: 31207575 |
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Reproductive Biology and Endocrinology |
Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in … Pubmed: 31311554 |
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Experimental Neurology |
Reduced serotonin impairs long-term depression in basolateral amygdala complex and causes anxiety-like behaviors in a mouse model of perimenopause Pubmed: 31377402 |
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Oxidative medicine and cellular longevity |
Total Polysaccharides of Lily Bulb Ameliorate Menopause-Like Behavior in Ovariectomized Mice: Multiple Mechanisms Distinct from Estrogen Therapy Pubmed: 31428228 |
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Journal of Ovarian Research |
Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase Pubmed: 31722718 |
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Cellular Signalling |
Effect of FSH on E2/GPR30-mediated mouse oocyte maturation in vitro Pubmed: 31704004 |
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Chemosphere |
Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling Pubmed: 31809938 |
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Neuroscience |
Blocking of Estradiol Receptors ERα, ERβ and GPER During Development, Differentially Alters Energy Metabolism in Male and Female Rats Pubmed: 31805254 |
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Open Access Maced J Med Sc |
Gambir Extract (Uncaria Gambir) Decreases Inflammatory Response and Increases Gastric Mucosal Integrity in Wistar Rats-Model Gastritis Pubmed: 31949507 |
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Estradiol treatment during perinatal development alters Pubmed: 31966342 |
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science of the total environment |
Effects of fast food packaging plasticizers and their metabolites on steroid hormone synthesis in H295R cells Pubmed: 32334352 |
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molecular nutrition & food research |
Lepidiline A Improves the Balance of Endogenous Sex Hormones and Increases Fecundity by Targeting HSD17B1 Pubmed: 32239630 |
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Chemosphere |
Prenatal exposure of female mice to perfluorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21 … Pubmed: 33131727 |
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY |
Parental exposure to polystyrene microplastics at environmentally relevant concentrations has negligible transgenerational effects on zebrafish (Danio rerio) Pubmed: 33011509 |
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Neuropharmacology |
Impaired LTD-like motor cortical plasticity in female patients with major depression disorder Pubmed: 32791084 |
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JOURNAL OF INTERNATIONAL MEDICAL RESEARCH |
Protective effects of irbesartan and benazepril against vaginal vascular remodeling and fibrosis in female spontaneously hypertensive rats Pubmed: 32790534 |
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ARCHIVES OF GYNECOLOGY AND OBSTETRICS |
MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance Pubmed: 32757043 |
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Antioxidants (Basel) |
Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal Model Pubmed: 32517356 |
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Andrologia |
Maternal CBZ exposure impairs testicular development, spermatogenesis and sperm parameters in male offspring at puberty Pubmed: 32478927 |
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Neurotoxicology |
Chronic exposure to low dose of bisphenol A causes follicular atresia by inhibiting kisspeptin neurons in anteroventral periventricular nucleus in female mice Pubmed: 32407858 |
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archives of toxicology |
Effect of estrogen-active compounds on the expression of RACK1 and immunological implications Pubmed: 32328699 |
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Heliyon |
Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting … Pubmed: 33005806 |
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BioMed Research International |
Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress Pubmed: 32685488 |
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Iranian Journal of Basic Medical Sciences |
Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS |
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biorxiv |
The effects of human umbilical cord mesenchymal stem cell transplantation on female fertility preservation in mice |
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Moosense pedometer activity and periestrual hormone profile in relation to oestrus in crossbred cattle |
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J Steroid Biochem Mol Biol |
Dihydrotestosterone regulates oestrogen secretion, oestrogen receptor expression, and apoptosis in granulosa cells during antral follicle development 33465420 |
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JOURNAL OF ETHNOPHARMACOLOGY |
Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis 33422655 |
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JOURNAL OF ETHNOPHARMACOLOGY |
BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-¦ÊB pathways in aging mice induced by D-galactose 33508368 |
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Chronic Exposure to Perfluorohexane Sulfonate Leads to a Reproduction Deficit by Suppressing Hypothalamic Kisspeptin Expression in Mice |
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Comparison of ovarian striation and ovarian fragmentation in a rat model of ovarian insufficiency |
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Arch Gynecol Obstet |
The relationship between transforming growth factor β superfamily members (GDF11 and BMP4) and lumbar spine bone mineral density in postmenopausal Chinese … 34417839 |
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J Virol |
Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice 34730391 |
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Stem Cells Int |
Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in … 34733331 |
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Eur J Pharmacol |
17β-Estradiol promotes angiogenesis of stria vascular in cochlea of C57BL/6J mice 34822791 |
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IBRO Neuroscience Reports |
Effects of Treadmill Exercise on Anxiety-like Behavior in Association with Changes in Estrogen Receptors ERα, ERβ and Oxytocin of C57BL/6J Female Mice 34746914 |
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Nutrients |
Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice Pubmed:35057459 |
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Nat Commun |
TrkB agonist antibody ameliorates fertility deficits in aged and cyclophosphamide-induced premature ovarian failure model mice Pubmed:35177657 |
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Appl Biochem Biotechnol |
ERα, but not ERβ and GPER, Mediates Estradiol-Induced Secretion of TSH in Mouse Pituitary Pubmed:35138554 |
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Stem Cell Research & Therapy |
G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and … |
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Research Square |
Drug-free in vitro activation combined 3D-bioprinted adipose-derived stem cells restore ovarian function of premature ovarian insufficiency |
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Catalog No. |
Related products for research use of Pan-species (General) Organism species |
Applications (RESEARCH USE ONLY!) |
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Immunogen; SDS-PAGE; WB. |
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CPA461Ge21 |
Immunogen; SDS-PAGE; WB. |
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CPA461Ge31 |
Immunogen; SDS-PAGE; WB. |
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PAA461Ge01 |
ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
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LAA461Ge71 |
WB; IHC; ICC. |
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LAA461Ge81 |
WB; IHC; ICC; IF. |
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MAA461Ge21 |
ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
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MAA461Ge28 |
ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
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MAA461Ge27 |
ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
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MEA461Ge |
Enzyme-linked immunosorbent assay for Antigen Detection. |
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CEA461Ge |
Enzyme-linked immunosorbent assay for Antigen Detection. |
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IEA461Ge |
Enzyme-linked immunosorbent assay for Antigen Detection. |
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CCA461Ge |
Chemiluminescent immunoassay for Antigen Detection. |
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LMA461Ge |
Magnetic Luminex Assay for Antigen Detection. |
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KSA461Ge11 |
Main materials for "Do It (ELISA Kit) Yourself". |
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