Skip to product information
1 of 1

Cloud-clone

SKU:LAA461Ge81

FITC-Linked Polyclonal Antibody to Estradiol (E2)

FITC-Linked Polyclonal Antibody to Estradiol (E2)

Regular price $445.00 USD
Regular price Sale price $445.00 USD
Sale Sold out
Shipping calculated at checkout.
UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

LAA461Ge81

Organism Species

Pan-species (General).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

FITC

Original Antibody

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IF.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against E2. It has been selected for its ability to recognize E2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

BioMed research international

Antiosteoclastic Activity of Milk Thistle Extract after Ovariectomy to Suppress Estrogen Deficiency-Induced Osteoporosis PubMed: PMC3678416

Fertility and Sterility

Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells Pubmed: 23954356

Repositório Digital

Utilizacao de células-tronco adultas para tratamento de falência ovariana causada por quimioterapia em camundongas Lume: Source

Electromagnetic Biology and Medicine

The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats Informahealthcare: Source

Academic Sciences

Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female rats Ijppsjournal: Source

Stem Cells Int.

Cell Therapy for Chemically Induced Ovarian Failure in Mice Pubmed:Pmc4274854

Reproductive Toxicology

Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring Pubmed:24126190

Phytomedicine.

Phloretin promotes osteoclast apoptosis in murine macrophages and inhibits estrogen deficiency-induced osteoporosis in mice Pubmed:24932975

Turkish Journal of Urology

Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy Turkishjournalofurology:Source

international journal of environmental research and public health

Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy PubMed: 25996886

Reprod Toxicol

In utero PubMed: 25530038

oncology research

Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats PubMed: 26629940

Environ Toxicol

Effect on the H19 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis period PubMed: 25782373

J Endocrinol

Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarction PubMed: 25770118

Reproduction

Prenatal and lactation nicotine exposure affects Sertoli cell and gonadotropin levels in rats PubMed: 26556892

Veterinary Word

Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers Pubmed:27051212

Archives of Physiology and Biochemistry

Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder Pubmed:26934364

Plos One

Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota Pubmed:27093642

Biology of Reproduction

Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring Pubmed:26962114

PPAR Research

Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction byUpreCavia (Guinea pig )lating PPARα Pubmed:27413362

Clinical and Experimental Hypertension

Assessment of ADMA, estradiol, and progesterone in severe preeclampsia Pubmed:27152507

PLoS Genet.

A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in Dendritogenesis Pubmed:26977770

Scientific Reports

The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilization pubmed:27876896

Scientific Reports

Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373

33

Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389

African Journal of Environmental Science and Technology

Pollution by endocrine disrupting estrogens in aquatic ecosystems in Morogoro urban and peri-urban areas in Tanzania 150659

Journal of Functional Foods

Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women S1756464617301561

environmental pollution

Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061

Environmental toxicology and pharmacology

Combined oral contraceptive and nitric oxide synthesis inhibition synergistically causes cardiac hypertrophy and exacerbates insulin resistance in female rats. pubmed:28376377

PPAR Research

Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα pubmed:27413362

Toxicological Sciences

Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384

Sci Rep

Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. pubmed:28322267

Interact Cardiovasc Thorac Surg

Acute administration of oestradiol or progesterone in a spinal cord ischaemia–reperfusion model in rats pubmed:29049608

Toxicological Sciences

Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction pubmed:28973666

Reproductive Sciences

Abnormality of Klotho Signaling Is Involved in Polycystic Ovary Syndrome pubmed:28673204

Oncotarget

Rcan2 and estradiol independently regulate body weight in female mice pubmed:28624805

African Journal of Environmental Science and Technology

Pollution by endocrine disrupting estrogens in aquatic ecosystems in Morogoro urban and peri-urban areas in Tanzania 10.5897/AJEST2016.2234

Journal of Orthopaedic Research

The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice pubmed:29058779

Animal Production Science

Can serum levels of anti-Müllerian hormone and oestradiol in juvenile gilts be used to predict future reproductive performance? 10.1071/ANv57n12Ab065

Asian Journal of Biomedical and Pharmaceutical Sciences

A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/ò-catenin Signaling Pathways in Ovariectomized Rats ISSN 2249 – 622X

Nanotheranostics

The biocompatibility studies of polymer dots on pregnant mice and fetuses pubmed:29071192

DARU Journal of Pharmaceutical Sciences

Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway pubmed:29020999

Reproduction.

Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring pubmed:28687593

Molecular Medicine Reports

Molecular mechanism and functional role of macrophage colony‑stimulating factor in follicular granulosa cells pubmed:28656272

Annals of Rheumatic Diseases 

FRI0390 Impaired ovarian reserve in patients affected by systemic lupus erythematosus: a case-control study Suppl_2:728.1.abstract

Stem Cell Research & Therapy

The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity Pubmed:30021657

Environmental Pollution

Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625

Toxicological Sciences

Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons 10.1093:toxsci

Frontiers in Molecular Neuroscience

Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355

Aquatic Toxicology

Exposure to tris (1, 3-dichloro-2-propyl) phosphate for Two generations decreases fecundity of zebrafish at environmentally relevant concentrations Pubmed:29775925

Journal of Ethnopharmacology

Effects of water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat model Pubmed:29705515

Inflammation

Differential Effects of Brain Death on Rat Microcirculation and Intestinal Inflammation: Female Male Pubmed:29737476

European Journal of Neuroscience

Postnatal separation prevents the development of prenatal stress‐induced anxiety in association with changes in oestrogen receptor and oxytocin immunoreactivity in … Pubmed:29205599

Journal of Ovarian Research

DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway Pubmed:29321035

Medical Science Monitor

Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian … Pubmed:29674607

Biomedicine & Pharmacotherapy

Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons Doi: 10.1016/j.biopha.2018.08.135

Journal of Endocrinology

Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome Pubmed: 29535146

Stem Cell Research & Therapy

Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the … Pubmed: 30606243

journal of molecular neuroscience

Possible Metabolic Alterations among Autistic Male Children: Clinical and Biochemical Approaches Pubmed: 30600432

Heliyon

Establishment and evaluation of a novel mouse model of peri/postmenopausal depression Doi: 10.1016/j.heliyon.2019.e01195

Experimental and Therapeutic Medicine

Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary

Evidence-Based Complementary and Alternative Medicine

iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine

Biomedicine & Pharmacotherapy

G protein-coupled estrogen receptor activation improves contractile and diastolic functions in rat renal interlobular artery to protect against renal ischemia … Pubmed: 30784936

Biomedicine & Pharmacotherapy

Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve

Biology of Reproduction

G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Accelerates Restoration of Ovarian Function in Cyclophosphamide … Pubmed: 31034039

Biomedicine & pharmacotherapy

Aqueous extract of lily bulb ameliorates menopause-like behavior in ovariectomized mice with novel brain-uterus mechanisms distinct from estrogen therapy Pubmed: 31207575

Reproductive Biology and Endocrinology

Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in … Pubmed: 31311554

Experimental Neurology

Reduced serotonin impairs long-term depression in basolateral amygdala complex and causes anxiety-like behaviors in a mouse model of perimenopause Pubmed: 31377402

Oxidative medicine and cellular longevity

Total Polysaccharides of Lily Bulb Ameliorate Menopause-Like Behavior in Ovariectomized Mice: Multiple Mechanisms Distinct from Estrogen Therapy Pubmed: 31428228

Journal of Ovarian Research

Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase Pubmed: 31722718

Cellular Signalling

Effect of FSH on E2/GPR30-mediated mouse oocyte maturation in vitro Pubmed: 31704004

Chemosphere

Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling Pubmed: 31809938

Neuroscience

Blocking of Estradiol Receptors ERα, ERβ and GPER During Development, Differentially Alters Energy Metabolism in Male and Female Rats Pubmed: 31805254

Open Access Maced J Med Sc

Gambir Extract (Uncaria Gambir) Decreases Inflammatory Response and Increases Gastric Mucosal Integrity in Wistar Rats-Model Gastritis Pubmed: 31949507


Estradiol treatment during perinatal development alters Pubmed: 31966342

science of the total environment

Effects of fast food packaging plasticizers and their metabolites on steroid hormone synthesis in H295R cells Pubmed: 32334352

molecular nutrition & food research

Lepidiline A Improves the Balance of Endogenous Sex Hormones and Increases Fecundity by Targeting HSD17B1 Pubmed: 32239630

Chemosphere

Prenatal exposure of female mice to perfluorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21 … Pubmed: 33131727

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

Parental exposure to polystyrene microplastics at environmentally relevant concentrations has negligible transgenerational effects on zebrafish (Danio rerio) Pubmed: 33011509

Neuropharmacology

Impaired LTD-like motor cortical plasticity in female patients with major depression disorder Pubmed: 32791084

JOURNAL OF INTERNATIONAL MEDICAL RESEARCH

Protective effects of irbesartan and benazepril against vaginal vascular remodeling and fibrosis in female spontaneously hypertensive rats Pubmed: 32790534

ARCHIVES OF GYNECOLOGY AND OBSTETRICS

MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance Pubmed: 32757043

Antioxidants (Basel)

Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal Model Pubmed: 32517356

Andrologia

Maternal CBZ exposure impairs testicular development, spermatogenesis and sperm parameters in male offspring at puberty Pubmed: 32478927

Neurotoxicology

Chronic exposure to low dose of bisphenol A causes follicular atresia by inhibiting kisspeptin neurons in anteroventral periventricular nucleus in female mice Pubmed: 32407858

archives of toxicology

Effect of estrogen-active compounds on the expression of RACK1 and immunological implications Pubmed: 32328699

Heliyon

Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting … Pubmed: 33005806

BioMed Research International

Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress Pubmed: 32685488

Iranian Journal of Basic Medical Sciences

Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS

biorxiv

The effects of human umbilical cord mesenchymal stem cell transplantation on female fertility preservation in mice


Moosense pedometer activity and periestrual hormone profile in relation to oestrus in crossbred cattle

J Steroid Biochem Mol Biol

Dihydrotestosterone regulates oestrogen secretion, oestrogen receptor expression, and apoptosis in granulosa cells during antral follicle development 33465420

JOURNAL OF ETHNOPHARMACOLOGY

Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis 33422655

JOURNAL OF ETHNOPHARMACOLOGY

BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-¦ÊB pathways in aging mice induced by D-galactose 33508368


Chronic Exposure to Perfluorohexane Sulfonate Leads to a Reproduction Deficit by Suppressing Hypothalamic Kisspeptin Expression in Mice


Comparison of ovarian striation and ovarian fragmentation in a rat model of ovarian insufficiency

Arch Gynecol Obstet

The relationship between transforming growth factor β superfamily members (GDF11 and BMP4) and lumbar spine bone mineral density in postmenopausal Chinese … 34417839

J Virol

Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice 34730391

Stem Cells Int

Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in … 34733331

Eur J Pharmacol

17β-Estradiol promotes angiogenesis of stria vascular in cochlea of C57BL/6J mice 34822791

IBRO Neuroscience Reports

Effects of Treadmill Exercise on Anxiety-like Behavior in Association with Changes in Estrogen Receptors ERα, ERβ and Oxytocin of C57BL/6J Female Mice 34746914

Nutrients

Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice Pubmed:35057459

Nat Commun

TrkB agonist antibody ameliorates fertility deficits in aged and cyclophosphamide-induced premature ovarian failure model mice Pubmed:35177657

Appl Biochem Biotechnol

ERα, but not ERβ and GPER, Mediates Estradiol-Induced Secretion of TSH in Mouse Pituitary Pubmed:35138554

Stem Cell Research & Therapy

G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and …

Research Square

Drug-free in vitro activation combined 3D-bioprinted adipose-derived stem cells restore ovarian function of premature ovarian insufficiency


Catalog No.

Related products for research use of Pan-species (General) Organism species

Applications (RESEARCH USE ONLY!)

CPA461Ge11

BSA Conjugated Estradiol (E2)

Immunogen; SDS-PAGE; WB.

CPA461Ge21

OVA Conjugated Estradiol (E2)

Immunogen; SDS-PAGE; WB.

CPA461Ge31

KLH Conjugated Estradiol (E2)

Immunogen; SDS-PAGE; WB.

PAA461Ge01

Polyclonal Antibody to Estradiol (E2)

ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).

LAA461Ge71

Biotin-Linked Polyclonal Antibody to Estradiol (E2)

WB; IHC; ICC.

LAA461Ge81

FITC-Linked Polyclonal Antibody to Estradiol (E2)

WB; IHC; ICC; IF.

MAA461Ge21

Monoclonal Antibody to Estradiol (E2)

ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).

MAA461Ge28

Monoclonal Antibody to Estradiol (E2)

ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).

MAA461Ge27

Monoclonal Antibody to Estradiol (E2)

ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).

MEA461Ge

Mini Samples ELISA Kit for Estradiol (E2)

Enzyme-linked immunosorbent assay for Antigen Detection.

CEA461Ge

ELISA Kit for Estradiol (E2)

Enzyme-linked immunosorbent assay for Antigen Detection.

IEA461Ge

Instant ELISA Kit for Estradiol (E2)

Enzyme-linked immunosorbent assay for Antigen Detection.

CCA461Ge

CLIA Kit for Estradiol (E2)

Chemiluminescent immunoassay for Antigen Detection.

LMA461Ge

Magnetic Luminex Assay Kit for Estradiol (E2) ,etc.

Magnetic Luminex Assay for Antigen Detection.

KSA461Ge11

ELISA Kit DIY Materials for Estradiol (E2)

Main materials for "Do It (ELISA Kit) Yourself".

 

View full details