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SKU:LAS099Ge81

FITC-Linked Polyclonal Antibody to Sialic Acid (SA)

FITC-Linked Polyclonal Antibody to Sialic Acid (SA)

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UOM
Concentration
Organism species

Western blotting: 0.5-2ug/ml
;Immunocytochemistry in formalin fixed cells: 5-20ug/ml
;Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
;Immunohistochemistry in paraffin section: 5-20ug/ml
;Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
;Optimal working dilutions must be determined by end user.

Product No.

LAS099Ge81

Organism Species

Pan-species (General).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

FITC

Original Antibody

CPS099Ge11-BSA Conjugated Sialic Acid (SA)

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

Pan-species (General)

Applications

WB; IHC; ICC; IF.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against SA. It has been selected for its ability to recognize SA in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

PLOS ONE

Broad Spectrum Activity of a Lectin-Like Bacterial Serine Protease Family on Human Leukocytes Pubmed:25251283

MOLECULAR AND CELLULAR BIOCHEMISTRY

Ectopic expression of 35 kDa and knocking down of 78 kDa SG2NAs induce cytoskeletal reorganization, alter membrane sialylation, and modulate the markers of EMT Pubmed: 33083950

Exp Gerontol

Sialic acid associated with oxidative stress and total antioxidant capacity (TAC) expression level as a predictive indicator in moderate to severe Alzheimer's disease Pubmed: 32991981


Catalog No.

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Applications (RESEARCH USE ONLY!)

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WB; IHC; ICC; IF.

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Main materials for "Do It (ELISA Kit) Yourself".

 

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