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SKU:LAB979Hu81

FITC-Linked Polyclonal Antibody to Hepcidin (Hepc)

FITC-Linked Polyclonal Antibody to Hepcidin (Hepc)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

LAB979Hu81

Organism Species

Homo sapiens (Human).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

FITC

Original Antibody

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IF.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against Hepc. It has been selected for its ability to recognize Hepc in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

World J Gastroenterol.

Hepcidin expression in colon during trinitrobenzene sulfonic acid-induced colitis in rats Pubmed: 24764672

International Journal of Advanced Research

Modulations of serum hepcidin, IL6 and iron status by different exercise regimens in ovarectomized rats Com:Source

Neurochemical Journal

Serum iron metabolism markers including hepcidin in multiple sclerosis patients Springer:Source

Afro-Egypt J Infect Endem Dis

Monitoring Hepcidin Level in Chronic Hepatitis C Virus Patients during Therapy Zu:Source

Gastroenterology

Gluconeogenic signals regulate iron homeostasis via hepcidin in mice Pubmed:Pmc3989026

J Card Fail.

Inappropriate expression of hepcidin by liver congestion contributes to anemia and relative iron deficiency Pubmed:24440572

Food Funct.

The action of JAK, SMAD and ERK signal pathways on hepcidin suppression by polysaccharides from Angelica sinensis in rats with iron deficiency anemia Pubmed:24752529

Free Radic Res.

Changes in skeletal muscle iron metabolism outpace amyotrophic lateral sclerosis onset in transgenic rats bearing the G93A hmSOD1 gene mutation Pubmed:25175826

Saudi J Kidney Dis Transpl.

Hepcidin and regulation of iron homeostasis in maintenance hemodialysis patients Pubmed:25193892

J Endocrinol Invest.?

The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders Pubmed:25245337

Gastroenterology Research and Practice

An Evaluation of the Correlation between Hepcidin Serum Levels and Disease Activity in Inflammatory Bowel Disease Pubmed:25628652

Clin Lab.

Serum hepcidin levels in Bulgarian population. Pubmed:25651734

The Tohoku Journal of Experimental Medicine

Liver Congestion in Heart Failure Contributes to Inappropriately Increased Serum Hepcidin despite Anemia Pubmed:25742771

Cancer Res

Hepcidin regulation in prostate and its disruption in prostate cancer PubMed: 25858146

Biochem Biophys Res Commun

B-cell translocation gene 2 promotes hepatic hepcidin production via induction of Yin Yang 1 PubMed: 25839654

Blood Purif

Effect of a More Permeable Dialysis Membrane on ESA Resistance in Hemodialysis Patients - A Pilot Investigation PubMed: 26536083

Asian Journal of Clinical Nutrition

Urinary Hepcidin in Diagnosis of Iron Deficiency Anemia Openview: E01Ad7C4A9109Ed5Fadb03Ec206D7Bdc

Front Psychol

Ferrous sulfate, but not iron polymaltose complex, aggravates local and systemic inflammation and oxidative stress in dextran sodium sulfate-induced colitis in rats PubMed: 25852597

Biol Trace Elem Res

Iron Supplementation Attenuates the Inflammatory Status of Anemic Piglets by Regulating Hepcidin PubMed: 25774043

APS Journals

Kupffer cell depletion protects against the steatosis, but not the liver damage, induced by marginal copper, high fructose diet in male rats. PubMed: 25813056

European Journal of Haematology

Hepcidin in tumor‐related iron deficiency anemia and tumor‐related anemia of chronic disease: pathogenic mechanisms and diagnosis PubMed: 24954786

WJA

The Effects of Treatment on Serum Hepcidin and Iron Homeostasis in HIV-1-Infected In-dividuals Journal: 59107

Nutrition, Metabolism and Cardiovascular Diseases

Hepcidin and iron metabolism associated with cardiometabolic risk factors in children: A case-control study. Pubmed:27139516

Food Funct

The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice Pubmed:26757699

Antioxid Redox Signal.

Hydrogen sulfide attenuates inflammatory hepcidin by reducing IL-6 secretion and promoting SIRT1-mediated STAT3 deacetylation Pubmed:26154696

BioMed Research International

The Impact of Acute Matriptase Inhibition in Hepatic Inflammatory Models. pubmed:27642598

Frontiers in Neuroscience

In Absence of the Cellular Prion Protein, Alterations in Copper Metabolism and Copper-Dependent Oxidase Activity Affect Iron Distribution. pubmed:27729845

Experimental Biology and Medicine

Effects of iron supplementation in mice with hypoferremia induced by obesity pubmed:27439539

Nutrition, Metabolism and Cardiovascular Diseases

Hepcidin and iron metabolism associated with cardiometabolic risk factors in children: A case–control study pubmed:27139516

PLOS ONE

S-Propargyl-Cysteine, a Novel Hydrogen Sulfide Donor, Inhibits Inflammatory Hepcidin and Relieves Anemia of Inflammation by Inhibiting IL-6/STAT3 Pathway. pubmed:27649298

Endocrine connections

Hepcidin links gluco-toxicity to pancreatic beta cell dysfunction by inhibiting Pdx-1 expression pubmed:28179377


Maternal and cord blood hepcidin levels based on gestational weeks in term and preterm infants publication:305921433_Maternal_and_cord_blood_hepc

Oxidative Medicine and Cellular Longevity

Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation pubmed:29147463

The Royal Society of Chemistry

Silkworm feces extract improves iron deficiency anemia via suppressing hepcidin expression and promoting iron-regulatory proteins expression DOI: 10.1039/C7RA09576G

Journal of Inflammation

Evaluation of a competitive hepcidin ELISA assay in the differential diagnosis of iron deficiency anaemia with concurrent inflammation and anaemia of inflammation in elderly patients 10.1186:s12950-017-0166-3

Antioxidants and Redox Signaling

AMPK serves as a Therapeutic Target against Anemia of Inflammation pubmed:27923278

NutriciónHospitalaria

Micronutrient supplementation in gastric bypass surgery: prospective study on infl ammation and iron metabolism in premenopausal women 10.20960/nh.1162

The Japanese Society of Hematology

Hepcidin suppression in β‑thalassemia is associated with the down‑regulation of atonal homolog 8 pubmed:28405918

Tuberculosis

Modulation of iron status biomarkers in tuberculosis-diabetes co-morbidity 10.1016/j.tube.2017.11.011

Oman Medical Journal

The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus. pubmed:28584599

Cellular Physiology and Biochemistry

The Effects of Angelica Sinensis Polysaccharide on Tumor Growth and Iron Metabolism by Regulating Hepcidin in Tumor-Bearing Mice Pubmed:29843136

Cellular Microbiology 

inhibits ferroportin translation by modulating FBXL5‐IRP2 axis for its growth within host macrophages Pubmed:29470856

Journal of Histochemistry & Cytochemistry

Acute and Chronic Iron Overloading Differentially Modulates the Expression of Cellular Iron-homeostatic Molecules in Normal Rat Kidney Pubmed:29873589

Clinical and Experimental Medicine

Increased NGAL level associated with iron store in chronic kidney disease with anemia Pubmed:29909502

Hematology

Relationship between obesity and iron deficiency anemia: is there a role of hepcidin? Pubmed:29318945


Hepcidin serum levels in HCV chronically mono-infected naïve patients, compared to healthy individuals 77856

Food & Function

Effect of iron supplement from microalgae on the treatment of iron-deficient anemia in rats Doi: 10.1039/C8FO01696H

Haematologica

New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia Pubmed: 30792208

Ann Intensive Care

Inflammatory anemia-associated parameters are related to 28-day mortality in patients with sepsis admitted to the ICU: a preliminary observational study Pubmed: 31183575

Journal of Functional Foods

Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats

Naunyn Schmiedebergs Arch Pharmacol.

3-Amidinophenylalanine-derived matriptase inhibitors can modulate hepcidin production in vitro Pubmed: 31659405

DEVELOPMENT GROWTH & DIFFERENTIATION

H2O2 induces oxidative stress damage through the BMP‐6/SMAD/hepcidin axis Pubmed: 32012242

Frontiers in Physiology

Increased Hepcidin Levels During a Period of High Training Load Do Not Alter Iron Status in Male Elite Junior Rowers Pubmed: 32038278

J Korean Med Sci

Can Calprotectin Show Subclinical Inflammation in Familial Mediterranean Fever Patients? Pubmed: 32174062

nephrology dialysis transplantation

Iron kinetics following treatment with sucroferric oxyhydroxide or ferric citrate in healthy rats and models of anaemia, iron overload or inflammation Pubmed: 32259248

mikrochimica acta

Gold-silver core-shell nanoparticle–based impedimetric immunosensor for detection of iron homeostasis biomarker hepcidin Pubmed: 33095336

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY

Circulating Osteocyte‐Related Biomarkers (vitamin D, sclerostin, dickkopf-1), hepcidin, and oxidative stress markers in early breast cancer: their impact in … Pubmed: 33065276

Sleep Breath

Relation of serum hepcidin levels and restless legs syndrome in chronic hemodialysis patients Pubmed: 33029690

journal of trace elements in experimental medicine

Difference in iron metabolism may partly explain sex-related variability in the manifestation of Wilson's disease Pubmed: 32937238

reproduction in domestic animals

Expression of iron regulatory proteins in full term swine placenta Pubmed: 32449967

Neurobiology of Aging

Estrogen deficiency is associated with brain iron deposition via upregulation of hepcidin expression in aged female mice Pubmed: 32920472

The Egyptian Journal of Haematology

Urinary hepcidin concentration in assessment of iron homeostasis in pediatrics


COMPARATIVE MONITORING OF THE ENZYME ACTIVITY OF BONE METABOLISM IN PATIENTS AFTER DENTAL IMPLANTATION


Serum iron, prealbumin, D-dimer and prothrombin time are indicators of the diagnosis, severity and outcome of Salmonella enterica serovar Enteritidis infection


Гепсидин и индекс фиброза печени MELD-XI как маркеры полиорганной недостаточности у пациентов хронической сердечной недостаточностью с …


Ассоциации уровней NT-proBNP и гепсидина с клинико-лабораторными параметрами у больных хронической сердечной недостаточностью с разной …

Biosci Rep

Correlation of hepcidin and serum ferritin levels in thalassemia patients at Chiang Mai University Hospital 33565577

Biometals

Iron metabolism is disturbed and anti-copper treatment improves but does not normalize iron metabolism in Wilson's disease 33555495

J Mol Cell Cardiol

Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure 34139233


Continuous Veno-Venous Hemofiltration (CVVH) Improves Iron Metabolism Disorders in Patients With Sepsis: A Single-Center Prospective Cohort Study

Reports of Biochemistry & Molecular Biology

Evaluation of Serum Cyclooxygenase, Hepcidin Levels in Acute Renal Injury (AKI) Patients Following Cardiac Catheterization

Food Funct

High-dose ferric citrate supplementation attenuates omega-3 polyunsaturated fatty acid biosynthesis via downregulating delta 5 and 6 desaturases in rats with high-fat … 34787162

J Trace Elem Med Biol

Caffeine and alcohol-friends or foes of human iron stores? Pubmed:35063815

PeerJ

Resveratrol ameliorates iron overload induced liver fibrosis in mice by regulating iron homeostasis Pubmed:35698613


Serum Calprotectin and Iron Metabolism Biomarker Levels in Behçet's Disease

Food Bioscience

Roles of homopolymeric apoferritin in alleviating alcohol-induced liver injury


Catalog No.

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