Cloud-clone
SKU:LAB214Hu81
FITC-Linked Polyclonal Antibody to Aspartate Aminotransferase (AST)
FITC-Linked Polyclonal Antibody to Aspartate Aminotransferase (AST)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAB214Hu81
Organism Species
Homo sapiens (Human).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
FITC
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IF.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against AST. It has been selected for its ability to recognize AST in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Kidney International |
Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and -independent mechanisms PubMed: PMC3612362 |
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Proceedings of the National Academy of Sciences |
Pathogenesis of emerging severe fever with thrombocytopenia syndrome virus in C57/BL6 mouse model PubMed: PMC3382536 |
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African Journal of Pharmacy and Pharmacology |
Protective effect of Panax ginseng against N-acetyl-p-aminophenol-induced hepatotoxicity in rats Academicjournals: Source |
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Kidney International |
Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and-independent mechanisms Pubmed: 23325074 |
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European Journal of Pharmacology |
Protective effect of Et-1 receptor antagonist bosentan on paracetamol induced acute liver toxicity in rats ScienceDirect: S0014299914000235 |
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Journal of Immunology |
The P2X1 Receptor Is Required for Neutrophil Extravasation during Lipopolysaccharide-Induced Lethal Endotoxemia in Mice Pubmed:25480563 |
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Gastroenterol Res Pract |
Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning PubMed: 26697061 |
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Gastroenterol Res Pract. |
Advanced Studies in Clinical and Experimental Research in Gastroenterology Pubmed:26955389 |
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Scientific Reports |
Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. pubmed:27678302 |
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Biochimica et Biophysica Acta (BBA)-General Subjects |
Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats pubmed:28341485 |
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Scientific Reports |
Deletion of MCP-1 Impedes Pathogenesis of Acid Ceramidase Deficiency Pubmed:29379059 |
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FEBS Open Bio |
A critical role of hepatitis B virus polymerase in cirrhosis, hepatocellular carcinoma, and steatosis Pubmed:29321963 |
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BMC Complementary and Alternative Medicine |
Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents Pubmed: 28095910 |
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laboratory investigation |
Hepatic pathology and altered gene transcription in a murine model of acid ceramidase deficiency Pubmed: 31186526 |
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Brain Behavior and Immunity |
Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathy Pubmed: 31437533 |
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Biomed Research International |
Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat Diet Pubmed: 32149099 |
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Nanomaterials |
Biological Safety and Biodistribution of Chitosan Nanoparticles Pubmed: 32340313 |
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European Journal of Biology |
Effects of Tempol in Lipopolysaccharide-Induced Liver Injury |
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Pathogens |
Antimalarial Effect of the Total Glycosides of the Medicinal Plant, Ranunculus japonicus 33925018 |
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Int Immunopharmacol |
Taraxasterol mitigates Con A-induced hepatitis in mice by suppressing interleukin-2 expression and its signaling in T lymphocytes 34848154 |
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Acta Pharmacol Sin |
NFAT inhibitor 11R-VIVIT ameliorates mouse renal fibrosis after ischemia-reperfusion-induced acute kidney injury 34937917 |
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J Magn Reson Imaging |
Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver … Pubmed:35212074 |
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Medcomm |
Blockade of mIL‐6R alleviated lipopolysaccharide‐induced systemic inflammatory response syndrome by suppressing NF‐κB‐mediated Ccl2 expression and … Pubmed:35548710 |
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International Immunopharmacology |
Dimethyl fumarate ameliorates autoimmune hepatitis in mice by blocking NLRP3 inflammasome activation Pubmed:35605433 |
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Main materials for "Do It (ELISA Kit) Yourself". |
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