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SKU:LAA836Hu81
FITC-Linked Polyclonal Antibody to Adrenocorticotropic Hormone (ACTH)
FITC-Linked Polyclonal Antibody to Adrenocorticotropic Hormone (ACTH)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAA836Hu81
Organism Species
Homo sapiens (Human).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
FITC
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IF.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against ACTH. It has been selected for its ability to recognize ACTH in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Respir Res. |
Intracerebroventricular injection of leukotriene B4 attenuates antigen-induced asthmatic response via BLT1 receptor stimulating HPA-axis in sensitized rats. PubMed: 20403205 |
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Toxicology Letters |
Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats Pubmed:24440345 |
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Mol Med Rep. |
Ursolic acid suppresses growth and adrenocorticotrophic hormone secretion in AtT20 cells as a potential agent targeting adrenocorticotrophic hormone-producing pituitary adenoma. Pubmed:24682498 |
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Peptides |
Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level Pubmed:25236411 |
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Journal of Animal and Feed Sciences |
Interdependence of the peripheral metabolism of glucocorticoids and thyroid hormones under calorie deficit in rats at different ages Researchgate:Source |
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International Journal of Immunopathology & Pharmacology |
Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats PubMed: 26684638 |
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Cell Tissue Res |
CXCL10/CXCR3 signaling mediates inhibitory action by interferon-gamma on CRF-stimulated adrenocorticotropic hormone (ACTH) release PubMed: 26572542 |
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Neuroscience Letters |
Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depression Pubmed:26773866 |
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Neural Regeneration Research |
Moxibustion upreCavia (Guinea pig )lates hippocampal progranulin expression. Pubmed:27212922 |
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Environmental Science and Pollution Research |
Lycopene ameliorates atrazine-induced oxidative damage in adrenal cortex of male rats by activation of the Nrf2/HO-1 pathway Pubmed:27102619 |
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Sci Rep. |
Hedgehog signaling activation induces stem cell proliferation and hormone release in the adult pituitary gland Pubmed:27109116 |
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Sci Rep. |
High activity of the stress promoter contributes to susceptibility to stress in the tree shrew Pubmed:27125313 |
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brain research bulletin |
Activation of ATP-sensitive potassium channel by iptakalim normalizes stress-induced HPA axis disorder and depressive behaviour by alleviating inflammation and oxidative stress in mouse hypothalamus. pubmed:28161195 |
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中国临床药理学与治疗学 |
肉蔻五味丸抗抑郁作用及机制研究 9787 |
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Neuroscience |
Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation pubmed:28663093 |
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Toxicology |
Mixture effects of azole fungicides on the adrenal gland in a broad dose range pubmed:28454766 |
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Biomedicine & Pharmacotherapy |
Triptolide suppresses growth and hormone secretion in murine pituitary corticotroph tumor cells via NF-kappaB signaling pathway pubmed:28892788 |
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Hormones and Behavior |
Acth-induced model of depression resistant to tricyclic antidepressants: Neuroendocrine and behavioral changes and influence of long-term magnesium … Pubmed:30025718 |
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Frontiers in Pharmacology |
Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary … Pubmed:30038568 |
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Molecular and Cellular Endocrinology |
Metformin suppresses growth and adrenocorticotrophic hormone secretion in mouse pituitary corticotroph tumor AtT20 cells 10.1016:j.mce.2018.07.007 |
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Frontiers in Behavioral Neuroscience |
Chronic hippocampal abnormalities and blunted HPA axis in an animal model of repeated unpredictable stress Pubmed:30079015 |
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Placenta |
Human placentophagy: Effects of dehydration and steaming on hormones, metals and bacteria in placental tissue Pubmed:29941176 |
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BMC Veterinary Research |
Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine … Pubmed:29540178 |
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European Journal of Applied Physiology |
Comparison between men and women of volume regulating hormones and aquaporin-2 excretion following graded central hypovolemia Pubmed: 30564880 |
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Cyber Leninka |
ТЭС-ТЕРАПИЯ КАК МЕТОД ПРЕДУПРЕЖДЕНИЯ ДЕЗАДАПТАЦИИ У САМЦОВ КРЫС С ВЫСОКОЙ СТРЕССОУСТОЙЧИВОСТЬЮ |
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Serbian Journal of Experimental and Clinical Research |
Assessment of the TDCS Influence on Stress-Induced Disorders in Rats with Low Stress Sustainability and Endurance |
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Scientific Reports |
Mice lacking Casp1, Ifngr and Nos2 genes exhibit altered depressive-and anxiety-like behaviour, and gut microbiome composition Pubmed: 31015500 |
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alcoholism-clinical and experimental research |
Persistent changes in stress‐regulatory genes in pregnant woman or a child with prenatal alcohol exposure Pubmed: 31329297 |
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Hormones and Behavior |
Hypothalamus-pituitary-adrenal axis imbalance and inflammation contribute to sex differences in separation-and restraint-induced depression Pubmed: 32165183 |
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Prog Neuropsychopharmacol Biol Psychiatry |
Progress in Neuropsychopharmacology & Biological Psychiatry Pubmed: 17134810 |
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Evid Based Complement Alternat Med |
So-Ochim-Tang-Gamibang, a Traditional Herbal Formula, Ameliorates Depression by Regulating Hyperactive Glucocorticoid Signaling In Vitro and In Vivo Pubmed: 33224257 |
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PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY |
Repeated unpredictable stress and social isolation induce chronic HPA axis dysfunction and persistent abnormal fear memory Pubmed: 32682873 |
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Aging Cell |
Altered glucocorticoid metabolism represents a feature of macroph‐aging Pubmed: 32463582 |
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neurochemical research |
Anxiolytic and Anti‑depressive Like Effects of Translocator Protein (18 kDa) Ligand YL‑IPA08 in a Rat Model of Postpartum Depression Pubmed: 32383026 |
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Behavioral Ecology and Sociobiology |
Exposure to eagle owl feces induces anti-predator behavior, physiology, and hypothalamic gene responses in a subterranean rodent, the plateau zokor (Eospalax … |
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EUROPEAN CHILD & ADOLESCENT PSYCHIATRY |
Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡ 33389158 |
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International Journal of?Nanomedicine |
Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡ 33762821 |
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J Am Heart Assoc |
Post©\Cardiac Arrest Hydrocortisone Use Ameliorates Cardiac Mitochondrial Injury in a Male Rat Model of Ventricular Fibrillation Cardiac Arrest 33960200 |
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Effects of Propofol versus Sevoflurane on Postoperative Pain and Neuroendocrine Stress Response in Oocyte Pickup Patients 33936350 |
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Main materials for "Do It (ELISA Kit) Yourself". |
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