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SKU:LAA702Hu81

FITC-Linked Polyclonal Antibody to Thioredoxin (Trx)

FITC-Linked Polyclonal Antibody to Thioredoxin (Trx)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

LAA702Hu81

Organism Species

Homo sapiens (Human).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

FITC

Original Antibody

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

Mus musculus (Mouse), Rattus norvegicus (Rat)

Applications

WB; IHC; ICC; IF.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against Trx. It has been selected for its ability to recognize Trx in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Human Reproduction

The roles of thioredoxin and thioredoxin-binding protein-2 in endometriosis OxfordJournals: source

Exp Ther Med.

Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosus Pubmed:Pmc4061188

Reproduction

Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis Pubmed:25376627

Free Radical Research

Thalassemia major patients using iron chelators showed a reduced plasma thioredoxin level and reduced thioredoxin reductase activity, despite elevated oxidative stress Pubmed:25564095

Antioxidants

Comparison of the Pulmonary Oxidative Stress Caused by Intratracheal Instillation and Inhalation of NiO Nanoparticles when Equivalent Amounts of NiO Are Retained in the Lung. Pubmed:26797643

FASEB Journal

Relationship between aortic wall oxidative stress/proteolytic enzyme expression and intraluminal thrombus thickness indicates a novel pathomechanism in the … Pubmed: 30351992

World Journal of Gastrointestinal Oncology

Redox, immune and genetic biomarker system for personalized treatments in colorectal cancer Doi: 10.4251/wjgo.v11.i2.117

Nutrients

The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes Pubmed: 30736479

European Journal of Pharmacology

Nilotinib enhances β-islets integrity and secretory functions in a rat model of STZ-induced diabetes mellitus Pubmed: 31351037

Journal of Clinical Medicine

Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort Pubmed: 32210109

HISTOCHEMISTRY AND CELL BIOLOGY

Thioredoxin 1 is upregulated in the bone and bone marrow following experimental myocardial infarction: evidence for a remote organ response Pubmed: 33161477

Journal of Cosmetic Dermatology

Examination of androgenetic alopecia with serum biomarkers Pubmed: 32969583

Heliyon

Hepatoprotective Effect of Saccharomyces Cervisciae Cell Wall Extract against Thioacetamide-induced Liver Fibrosis in Rats 34159266

Hum Exp Toxicol

Carvacrol hinders the progression of hepatic fibrosis via targeting autotaxin and thioredoxin in thioacetamide-induced liver fibrosis in rat 34155936


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