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SKU:LAA605Hu81

FITC-Linked Polyclonal Antibody to Adiponectin (ADPN)

FITC-Linked Polyclonal Antibody to Adiponectin (ADPN)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

LAA605Hu81

Organism Species

Homo sapiens (Human).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

FITC

Original Antibody

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IF.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ADPN. It has been selected for its ability to recognize ADPN in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

European Journal of Pharmacology

Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet PubMed: 20869961

BioMed Research International

Serum Fetuin-A Levels Related with Microalbuminuria in Diet-Induced Obese Rats PubMed: PMC3591142

PLoS One

CD40L Deficiency Attenuates Diet-Induced Adipose Tissue Inflammation by Impairing Immune Cell Accumulation and Production of Pathogenic IgG-Antibodies PubMed: PMC3297623

Molecular and Cellular Endocrinology

Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs ScienceDirect: S0303720712004194

British Journal of Nutrition

n-6: n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigs Pubmed: 23947577

International Urology Nephrology

Perirenal fat associated with microalbuminuria in obese rats Pubmed: 24526332

Int J Mol Sci.

Maternal high folic acid supplement promotes glucose intolerance and insulin resistance in male mouse offspring fed a high-fat diet. Pubmed: 24736781

Mol Biol Rep.

Adiponectin decreases lipids deposition by p38 MAPK/ATF2 signaling pathway in muscle of broilers. Pubmed: 24178343

Endocr J.

CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes Pubmed:25168658

Iran J Basic Med Sci

Zataria multiflora increases insulin sensitivity and PPARγ gene expression in high fructose fed insulin resistant rats Pubmed:Pmc4046234

Biometals.?

Perinatal low-dose iron treatment influences susceptibility to diet-induced adipogenesis in early-aged male Wistar rats Pubmed:24519243

Poult Sci.?

Effect of perches on liver health of hens Pubmed:24812236

Gen Comp Endocrinol.

The effect of recombinant adeno-associated virus-adiponectin (rAAV2/1-Acrp30) on glycolipid dysmetabolism and liver morphology in diabetic rats Pubmed:25019654

Journal of Trace Elements in Medicine and Biology

Adipose tissue chromium and vanadium disbalance in high-fat fed Wistar rats Pubmed:25194956

J Endocrinol

Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats Pubmed:25240061

Obesity Surgery

Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats Pubmed:25697126

Int J Clin Exp Pathol

Cold exposure stimulates lipid metabolism, induces inflammatory response in the adipose tissue of mice and promotes the osteogenic differentiation of BMMSCs via the p38 MAPK pathway PubMed: 26617802

Cell Physiol Biochem

Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos Pathway PubMed: 26160485

Life Sciences

Impaired adipogenesis in adipose tissue associated with hepatic lipid deposition induced by chronic inflammation in mice with chew diet PubMed: 26188592

MEDICAL RESEARCH JOURNAL

Relation between afamin level and some inflammatory markers in obese children Mrjnrc: Abstract


Swimming training attenuates the morphological reorganization of the myocardium and local inflammation in the left ventricle of growing rats with untreated experimental diabetes. Pubmed:26896925

Technologia Alimentaria

Decreased adipose tissue zinc content is associated with metabolic parameters in high fat fed Wistar rats abstract:10

Obes Surg.

Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in Obese Göttingen Minipig. Effects on Glucose Metabolism. Pubmed:27185175

Acta Histochemica

Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines science:S0065128116301234

Adv Biomed Res.

Trigonella foenum-graecum water extract improves insulin sensitivity and stimulates PPAR and γ gene expression in high fructose-fed insulin-resistant rats pmc:PMC4817393

Biological Trace Element Research

Early High-Fat Feeding Induces Alteration of Trace Element Content in Tissues of Juvenile Male Wistar Rats article:s12011-016-0777-1

Journal of Functional Foods

Antiobesity and antihyperlipidaemic effects of Yan-Sheng-Yin in animals and humans science:S1756464616300585

J Bone Miner Res

Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats Pubmed:26175082

Journal of Molecular and Cellular Cardiology

Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese mice pubmed:27638193

Journal of Diabetes Research

Central administration of galanin receptor 1 agonist boosted insulin sensitivity in adipose pubmed:27127795

Asian-Australasian Journal of Animal Sciences

Dietary Niacin Supplementation Suppressed Hepatic Lipid Accumulation in Rabbits pubmed:27004817

Biomedical and Pharmacology Journal

Association of ADIPOQ Gene Variant Rs266729 with Circulatory Adiponectin levels in Patients with type 2 Diabetes in North Indian Population: A Case- … vol10no1

IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES

Berberis integerrima ameliorates insulin resistance in high-fructose-fed insulin-resistant rats pubmed:29147484

Evidence-Based Complementary and Alternative Medicine

BuShenKangShuai tablet reduces atherosclerotic lesion by improving blood lipids metabolism and inhibiting inflammatory response via TLR4 and NF-κB signaling pathway journals:1758383

Oxidative Medicine and Cellular Longevity

Differential Susceptibility of Germ and Leydig Cells to Cadmium-Mediated Toxicity: Impact on Testis Structure, Adiponectin Levels, and Steroidogenesis journals:3405089

Iranian Journal of Pharmaceutical Research

Antihyperglycemic Effect of Rosa Damascena is Mediated by PPAR.γ Gene Expression in Animal Model of Insulin Resistance pubmed:29201096

Biomedical & Pharmacology Journal

Association of ADIPOQ Gene Variant Rs266729 with Circulatory Adiponectin levels in Patients with type 2 Diabetes in North Indian Population: A Case-Control Study DOI:10.13005/bpj/1123

Oncotarget

FAM3A enhances adipogenesis of 3T3-L1 preadipocytes via activation of ATP-P2 receptor-Akt signaling pathway pubmed:28515350

Diabetes & metabolism

Fenofibrate decreases plasma ceramide in type 2 diabetes patients: A novel marker of CVD? pubmed:28499696

Frontiers in Endocrinology

Circulating and Adipose Tissue mRNA Levels of Zinc-α2-Glycoprotein, Leptin, High-Molecular-Weight Adiponectin, and Tumor Necrosis Factor-Alpha in … Pubmed:29755407

Food & Function

Calycosin directly improves perivascular adipose tissue dysfunction by upregulating the adiponectin/AMPK/eNOS pathway in obese mice Pubmed:29595858

Evidence-based Complementary and Alternative Medicine

Bushenkangshuai Tablet Reduces Atherosclerotic Lesion by Improving Blood Lipids Metabolism and Inhibiting Inflammatory Response via TLR4 and NF-κB Signaling … Pubmed:29619063

Medicine (Baltimore)

Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity Pubmed:29953010

Veterinary immunology and immunopathology

Preactivation of neutrophils and systemic oxidative stress in dogs with hyperleptinemia Pubmed:30078593

Hematology

Relationship between obesity and iron deficiency anemia: is there a role of hepcidin? Pubmed:29318945

International Journal of Gerontology

Aging effects on circulating adiponectin levels and the expressions of adiponectin and adiponectin receptor 1 in the brains of male rats 10.1016:j.ijge.2018.01.002

Oncotarget

Epigallocatechin-3-gallate suppresses differentiation of adipocytes via regulating the phosphorylation of FOXO1 mediated by PI3K-AKT signaling in 3T3-L1 … Pubmed:29484120

J Bas Res Med Sci

Decreased level of the anti-inflammatory adipokines, secreted frizzled-related protein 5 and adiponectin, in high cholesterol diet-induced atherosclerotic rats article-1-352-en.pdf

Iraq Medical Journal

Adiponectin may be used as a marker in prediction of diabetic retinopathy patients JOCMS:Source

Food Science and Technology Research

Combinational Effect of Pine Needle Polysaccharide and Kudzu Flavonoids on Cell Differentiation and Fat Metabolism in 3T3-L1 Cells Doi: 10.3136/fstr.24.903

Journal of Pharmacological Sciences

Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome Pubmed: 30342783

Nutrients

Effects of Metformin Combined with Lactoferrin on Lipid Accumulation and Metabolism in Mice Fed with High-Fat Diet Pubmed: 30400147

Progress in Nutrition

Effect of fermented food consumption on biochemical parameters and adipokines levels Doi: 0.23751/pn.v20i4.7374

Biology of Reproduction

Recombinant adiponectin alleviates abortion in mice by regulating Th17/Treg imbalance via p38MAPK-STAT5 pathway Pubmed: 30496353

Nutrition in the Prevention and Treatment of Abdominal Obesity

Effects of Diet-Induced Early-Stage Obesity on a Low-Testosterone Gottingen Minipig

Evidence-Based Complementary and Alternative Medicine

BuShenKangShuai Tablet Alleviates Hepatic Steatosis via Improving Liver Adiponectin Resistance in ApoE-/-Mice

Life Sciences

Interrelation of liver vascularity to non-alcoholic fatty liver through a comparative study of the vasodilator effect of carvedilol or nicorandil in rats Pubmed: 30826497

Arteriosclerosis, Thrombosis, and Vascular Biology

Cardiac Lymphatic Dysfunction Causes Drug-Eluting Stent–Induced Coronary Hyperconstricting Responses in Pigs In Vivo Pubmed: 30816801


Modulation of the somatotropic axis, adiponectin and cytokine secretion during highly pathogenic porcine reproductive and respiratory syndrome virus type 1 (HP … Pubmed: 31003008

Gene

Expression of adipokines and their receptors in adipose tissue of women with class 3 obesity with or without hypertension Pubmed: 30940525

中国药理学通报

冷暴露对高脂饮食小鼠脂肪棕色化的影响

Nutrients

High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …

Proceedings of the Royal Society B: Biological Sciences

Paternal chronic folate supplementation induced the transgenerational inheritance of acquired developmental and metabolic changes in chickens Pubmed: 31506054

Cytokine

Increased plasma osteopontin levels are associated with nonalcoholic fatty liver disease in patients with type 2 diabetes mellitus Pubmed: 31514105

Food & Function

Collaborative Effects of Chlorogenic Acid and Caffeine on Lipid Metabolism via AMPKα-LXRα/SREBP-1c Pathway in High-Fat Diet-Induced Obese Mice Pubmed: 31670366

journal of ethnopharmacology

Dietary supplementation of Morus nigra L. leaves decrease fat mass partially through elevating leptin-stimulated lipolysis in pig model Pubmed: 31756448

Annals of Nutrition and Metabolism

Serum Asprosin Concentrations Are Increased and Associated with Insulin Resistance in Children with Obesity Pubmed: 31775140

BMC Endocrine Disorders

The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children Pubmed: 31771561

Journal of Hazardous Materials

Triphenyl phosphate causes a sexually dimorphic metabolism dysfunction associated with disordered adiponectin receptors in pubertal mice Pubmed: 31796355

Experimental and Clinical Endocrinology & Diabetes

HOMA-Adiponectin Closely Associates with Cardiometabolic Risk Markers in Middle-Aged Indians with Metabolic Syndrome Pubmed: 32189318

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Cerasus humilis cherry polyphenol reduces high-fat diet-induced obesity in C57BL/6 mice by mitigating fat deposition, inflammation, and oxidation Pubmed: 32227855

Nature Communications

AdipoR1/AdipoR2 dual agonist recovers nonalcoholic steatohepatitis and related fibrosis via endoplasmic reticulum-mitochondria axis Pubmed: 33199780

Metabolites

Adipocytokines and Metabolic Syndrome in Patients with Schizophrenia Pubmed: 33066473

Ann Nutr Metab

Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity Pubmed: 33027789

aging

Resemblance and differences in dietary restriction nephroprotective mechanisms in young and old rats Pubmed: 32970613

Diabetes Metab Syndr Obes

Serum ZAG and Adiponectin Levels Were Closely Related to Obesity and the Metabolically Abnormal Phenotype in Chinese Population Pubmed: 32904655

DIABETOLOGIA

Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via … Pubmed: 32880686

J Endocrinol Invest

Serum glypican4 and glycosylphosphatidylinositol-specific phospholipase D levels are associated with adipose tissue insulin resistance in obese subjects with … Pubmed: 32816247

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Highly Branched RG-I Domain Enrichment are Indispensable for Pectin Mitigating Against High-Fat Diet-Induced Obesity Pubmed: 32633953

J Endocrinol Invest

Activation of central galanin receptor 2 mitigated insulin resistance in adipocytes of diabetic rats Pubmed: 32588381

ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY

The effect of nutrition and exercise training on irisin and semaphorin-3E levels in obese patients Pubmed: 32569480

Diabetes

Exercise-Induced Increases in Insulin Sensitivity After Bariatric surgery are Mediated by Muscle Extracellular Matrix Remodeling Pubmed: 32409493

Journal of Cellular and Molecular Medicine

Cooperative effects of galanin and leptin on alleviation of insulin resistance in adipose tissue of diabetic rats Pubmed: 32395890

The Egyptian Journal of Chest Diseases and Tuberculosis

Study of serum adiponectin and resistin in patients with obstructive sleep apnea

Biosciences Biotechnology Research Asia

Association of+ 10211T/G (Rs17846866) Variant of Adiponectin Gene With Type 2 Diabetes Mellitus

Computational Biology and Chemistry

In-silico therapeutic investigations of arjunic acid and arjungenin as an FXR agonist and validation in 3T3-L1 adipocytes

Eur J Nutr

The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren 33575861

Eur J Pediatr

Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren 33834274

Cell Metab

TMBIM1 is an inhibitor of adipogenesis and its depletion promotes adipocyte hyperplasia and improves obesity-related metabolic disease 34107313

Biomedical and Environmental Sciences

Decreased Plasma MANF Levels are Associated with Type 2 Diabetes 33766221

International Journal of Endocrinology

Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet?¡­ 34035808

International Journal of Pharmacy and Biological Sciences

Anti-Obesity Efficacy of Selected Plants Against Progesterone Induced Obesity

Journal of Functional Foods

Smilax china L. polyphenols alleviates obesity and inflammation by modulating gut microbiota in high fat/high sucrose diet-fed C57BL/6J mice

Indian Journal of Forensic Medicine & Toxicology

Altered Serum Marker of Adipokines Profile in Breast Cancer Women

PPAR Research

Activation of the Peroxisome Proliferator-Activated Receptors (PPAR-α/γ) and the Fatty Acid Metabolizing Enzyme Protein CPT1A by Camel Milk Treatment … 34306045

Br J Nutr

The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study 34348809

Phytomedicine

Smilax china L. flavonoid alleviates HFHS-induced inflammation by regulating the gut-liver axis in mice 34561124

Biofactors

Vitamin D3 enhances the effects of omega‐3 oils against metabolic dysfunction‐associated fatty liver disease in rat 34767670

Kaohsiung J Med Sci

Associations of imbalance of intestinal flora with severity of disease, inflammatory factors, adiponectin, and vascular endothelial function of hypertension patients 34672426

Nat Commun

GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity 34853313

Animals

Effects of Obesity on Adiponectin System Skin Expression in Dogs: A Comparative Study 34438765

Cell Death & Disease

Hepatic deficiency of selenoprotein S exacerbates hepatic steatosis and insulin resistance Pubmed:35347118

Diabetes Metabolic Syndrome and Obesity-Targets and Therapy

Serum and salivary adipokines in type 2 diabetes–Results of a pilot study in India Pubmed:35717896

BioMed Research International

Adiponectin Levels Are Associated with White Matter Lesions (WMLs) and Cognitive Impairment Pubmed:35087911


High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients

Scientific Reports

SARS-CoV2 Induced Biochemical Mechanisms in Liver Damage and Intestinal Lesions.


Catalog No.

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Applications (RESEARCH USE ONLY!)

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WB; IHC; ICC; IF.

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WB; IHC; ICC; IP.

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WB; IHC; ICC; IP.

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WB; IHC; ICC; IP.

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WB; IHC; ICC; IP.

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ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.

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ELISA Kit DIY Materials for Adiponectin (ADPN)

Main materials for "Do It (ELISA Kit) Yourself".

 

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