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SKU:LAA553Hu81

FITC-Linked Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)

FITC-Linked Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

LAA553Hu81

Organism Species

Homo sapiens (Human).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

FITC

Original Antibody

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IF.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against MMP9. It has been selected for its ability to recognize MMP9 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Journal of Bone and Joint Surgery

Experimental osteoarthritis induced by surgical realignment of the patella in BALB/c mice PubMed: 21119180

Journal of Surgical Research

Serum Levels of Matrix Metalloproteinase-2 as a Marker of Intimal Hyperplasia ScienceDirect: S0022480409001942

Journal of Proteome Research

The Biological Behaviors of Rat Dermal Fibroblasts Can Be Inhibited by High Levels of MMP9 ACS: pr2006395

Zahedan Journal of Research in Medical Sciences

The Effect of Acute Sub-Maximal Endurance Exercise on Serum Angiogenic Indices in Sedentary Men Zjrms: Source

Experimental & Translational Stroke Medicine

Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental stroke PubMed: 21679435

PLoS One

Downregulation of CD9 in Keratinocyte Contributes to Cell Migration via Upregulation of Matrix Metalloproteinase-9 PubMed: PMC3797697

PLoS ONE

A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source

Rheumatology International

Hypothermia induced by adenosine 5′-monophosphate attenuates early stage injury in an acute gouty arthritis rat model Pubmed: 23408150

Clinical and Investigative Medicine

The protective effect of dexmedetomidine on bupivacaine-induced sciatic nerve inflammation is mediated by mast cells Pubmed: 23544611

Acta Histochemica

RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-κB signaling pathway in trophoblast cells Pubmed: 24681113

International journal of colorectal disease

Expression and inhibition of matrix metalloproteinase (MMP)-8, MMP-9 and MMP-12 in early colonic anastomotic repair Pubmed: 23619615

Journal of Periodontology

Effects of Photodynamic Therapy on the Clinical and Gingival Crevicular Fluid Inflammatory Biomarkers in Chronic Periodontitis: A Split-Mouth Randomized Clinical Trial Joponline: 130464

Polski Przeglad Chirurgiczny

Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative course Pubmed: 23585206

Thromb Haemost.

Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655

Int J Clin Exp Med.

Celastrol may have an anti-atherosclerosis effect in a rabbit experimental carotid atherosclerosis model Pubmed:Pmc4132129

The Scientific World Journal

Pass-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in rats Pubmed:24701154

Journal of Perinatology

Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasion Pubmed:25078863

World J Emerg Med

Changes in biological behaviors of rat dermal fibroblasts induced by high expression of MMP9 Pubmed:25215164

PLOS ONE

Melissa officinalis Extract Inhibits Laser-Induced Choroidal Neovascularization in a Rat Model Pubmed:25314292

Stem Cells International

Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro Pubmed:25767513

Physiology and Pharmacology

A Study on the Serum Levels of Angiogenic Factors in Response to Acute Long-term Submaximal Exercise in Sedentary Men Phypha:Source

Mediators of Inflammation

Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse Journals: Mi

Journal of Biomaterials and Tissue Engineering

MicroRNA-506 Up-Regulation Inhibits Growth and Invasion in Non-Small Cell Lung Cancer Cells by Possibly Targeting Sphingosine Kinase 1 Content: Asp

PLOS

Adiponectin Attenuates Lung Fibroblasts Activation and Pulmonary Fibrosis Induced by Paraquat PubMed: 25945502

Biomedical Materials

In vivo PubMed: 25634132

Histol Histopathol

Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cells PubMed: 26254457

Int J Clin Exp Pathol

Up-regulation of microRNA-302a inhibited the proliferation and invasion of colorectal cancer cells by regulation of the MAPK and PI3K/Akt signaling pathways PubMed: 26191138

Hattori and Ishihara Stem Cell Research & Therapy

Altered protein secretions during interactions between adipose tissue-or bone marrow-derived stromal cells and inflammatory cells PubMed: 25884474

Neuropeptides

Melanocortin MC5 receptor agonists alleviate brain damage in abdominal compartment syndrome in the rat PubMed: 25616531

Veterinary Parasitology

Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis PubMed: 26412140

Canadian Journal of Physiology and Pharmacology

Resveratrol reduces matrix metalloproteinases and alleviates intrahepatic cholestasis of pregnancy in rats Doi: Abs

PLASTIC AND AESTHETIC RESEARCH

A comparative study to evaluate the effect of limited access dressing on diabetic ulcers Article: 2347-9264

Kyselov

Влияние диуретиков с антиальдостероновым действием на состояние межклеточного матрикса и раннее постинфарктное ремоделирование левого … Article: View

Оригинальные исследования / Original researches

матриксу та ранн Article: Viewfile

Int J Mol Sci

Evodiamine Attenuates PDGF-BB-Induced Migration of Rat Vascular Smooth Muscle Cells through Activating PPARγ PubMed: 26703570

Deletion Polymorphism and Chronic Obstructive Pulmonary Disease

ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial Cells Pubmed:27075970

Basic Research

CTGF upreCavia (Guinea pig )lation correlates with MMP-9 level in airway remodeling in a murine model of asthma Journal:27699

Journal of Cellular and Molecular Medicine

Chronic myelogenous leukaemia exosomes modulate bone marrow microenvironment through activation of epidermal growth factor receptor Pubmed:27196940

Journal of Stroke and Cerebrovascular Diseases

Epigallocatechin Gallate Extends the Therapeutic Window of Recombinant Tissue Plasminogen Activator Treatment in Ischemic Rats Pubmed:26851971

Journal of the Science of Food and Agriculture

Actein protects against methylglyoxal-induced oxidative damage in osteoblastic MC3T3-E1 cells Pubmed:26991449

Cell Physiol Biochem.

MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1 Pubmed:27160777

Int Wound J.

From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748

Onco Targets Ther.

sequential measurements of serum matrix metalloproteinase 9 to monitor chemotherapy responses in patients with advanced non-small-cell lung cancer pmc:PMC4898419

Am J Pathol. 

Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy. Pubmed:26963343

Biochem Pharmacol.

Gallium, a promising candidate to disrupt the vicious cycle driving osteolytic metastases. pubmed:27378505

disease markers

Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases. pubmed:28348451

Balkan Medical Journal

Anti-inflammatory and Anti-apoptotic Effect of Valproic Acid and Doxycycline Independent from MMP Inhibition in Early Radiation Damage pubmed:27761275

SpringerPlus

The effect of long-term amiodarone administration on myocardial fibrosis and evolution of left ventricular remodeling in a porcine model of ischemic … pubmed:27652141

Cerebrovascular Diseases Extra

Increased Plasma Matrix Metalloproteinase-9 Levels Contribute to Intracerebral Hemorrhage during Thrombolysis after Concomitant Stroke and Influenza Infection pubmed:27560521

Microvascular Research

Pleiotropic effects of survivin in vascular endothelial cells. pubmed:27373518

BMC Veterinary Research

Metalloproteinases and their inhibitors are influenced by inhalative glucocorticoid therapy incombination with environmental dust reduction in equine recurrent airway obstruction. pubmed:27938355

Cellular Physiology and Biochemistry

MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1. pubmed:27160777

Journal of Biomaterials and Tissue Engineering

MicroRNA-340 Targets NF-κB1 to Inhibit Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma Cell Line publication:306067745

J Sci Food Agric.

Actein protects against methylglyoxal-induced oxidative damage in osteoblastic MC3T3-E1 cells. pubmed:26991449

Sci Total Environ.

The effects of particulate matter on inflammation of respiratory system: Differences betweenmale and female. pubmed:28174048

Journal of Biomaterials and Tissue Engineering

Zoledronic Acid Inhibits TGF-β1-Induced the Proliferation, Migration and EMT Through Smad Pathway in Human Gingival Fibroblasts art00008

Human cell

Liver X receptor α (LXRα) promoted invasion and EMT of gastric cancer cells by regulation of NF-κB activity pubmed:28091828

Human Cell

MicroRNA-124 upregulation inhibits proliferation and invasion of osteosarcoma cells by targeting sphingosine kinase 1 pubmed:27743351

Experimental Parasitology

Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis pubmed:28821422

Human Cell

MicroRNA-30a functions as tumor suppressor and inhibits the proliferation and invasion of prostate cancer cells by down-regulation of SIX1 pubmed:28573504

Cellular Physiology and Biochemistry

The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cells pubmed:29224019

Evidence-Based Complementary and Alternative Medicine

Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathway pubmed:28698735

International Wound Journal

From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases pubmed:26991748

Fundamental & Clinical Pharmacology

Epigallocatechin-3-gallate ameliorates intrahepatic cholestasis of pregnancy by inhibiting matrix metalloproteinase-2 and matrix metalloproteinase-9 DOI:10.1111/fcp.12294

Scientific Reports

Alantolactone induces apoptosis, promotes STAT3 glutathionylation and enhances chemosensitivity of A549 lung adenocarcinoma cells to doxorubicin via oxidative stress doi:10.1038/s41598-017-06535-y

Scientific Reports

Amphiregulin contained in NSCLC-exosomes induces osteoclast differentiation through the activation of EGFR pathway pubmed:28600504

Archives of Medical Science

CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma. pubmed:28507585

Molecular Medicine Reports

Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen‑induced arthritis pubmed:28901438

Surgical Innovation

A Time Course of Bevacizumab (Anti-VEGF) Effect on Rat Peritoneum: Relations Between Antiadhesive Action and Fibrin Regulation Enzymes. pubmed:28877644

Journal of Neurochemistry

Liraglutide ameliorated peripheral neuropathy in diabetic rats: Involvement of oxidative stress, inflammation and extracellular matrix remodeling Pubmed:29572844

Journal of Cellular Biochemistry

Matrix metalloproteinases‐2 (MMP‐2) and matrix metalloproteinases‐9 (MMP‐9) are differentially expressed in different regions of normal and preeclampsia … 10.1002:jcb.26849

Inflammation

Extracellular Matrix Remodeling and Modulation of Inflammation and Oxidative Stress by Sulforaphane in Experimental Diabetic Peripheral Neuropathy Pubmed:29704151

OncoTargets and Therapy

sirT4 acts as a tumor suppressor in gastric cancer by inhibiting cell proliferation, migration, and invasion Pubmed:30022839

Biomed Research International

CD30 Is Highly Expressed in Chronic Obstructive Pulmonary Disease and Induces the Pulmonary Vascular Remodeling Pubmed:29984229

Oncology Letters

miRNA‑199a‑5p suppresses proliferation and invasion by directly targeting NF‑κB1 in human ovarian cancer cells 10.3892:ol.2018.9170

Taiwanese Journal of Obstetrics & Gynecology

HMGB1-RAGE signaling pathway in pPROM Pubmed:29673663

Journal of Veterinary Science

Clinical assessment after human adipose stem cell transplantation into dogs Pubmed:29284215

Life Sciences

Inhibition of SRC/FAK cue: A novel pathway for the synergistic effect of rosuvastatin on the anti-cancer effect of dasatinib in hepatocellular carcinoma Pubmed: 30292831

Pediatric Research

Anti-thymic stromal lymphopoietin antibody suppresses airway remodeling in asthma through reduction of MMP and CTGF. Pubmed: 30464333

Journal of cellular physiology

Targeting peptidylarginine deiminase reduces neutrophil extracellular trap formation and tissue injury in severe acute pancreatitis Pubmed: 30515825

Clinical and experimental pharmacology & physiology

A prospective, randomized trial of intravenous hydroxocobalamin versus norepinephrine or saline for treatment of lipopolysaccharide‐induced hypotension in a swine … Pubmed: 30575111

Journal of Ocular Pharmacology and Therapeutics

Evaluation of the Effects of Biohcly in an Model of Mechanical Wounds in the Rabbit Cornea Pubmed: 30888249

Advanced Healthcare Materials

Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts Pubmed: 30838809

Cellular & Molecular Biology Letters

MiR-613 inhibits proliferation and invasion and induces apoptosis of rheumatoid arthritis synovial fibroblasts by direct down-regulation of DKK1

ARCHIVES OF ORAL BIOLOGY

Histopathological and Biochemical Evaluation of the effect of Paeoniflorin on the Periodontium During and After Periodontitis Formation in Rats Pubmed: 31005686


МАТРИКСНЫЕ МЕТАЛЛОПРОТЕИНАЗЫ: ЗНАЧЕНИЕ В РЕМОДЕЛИРОВАНИИ СЕРДЦА У ПАЦИЕНТОВ С ДИСПЛАЗИЕЙ СОЕДИНИТЕЛЬНОЙ ТКАНИ

Mediators of Inflammation

Metalloproteinases and their Inhibitors under the Course of Immunostimulation by CPG-ODN and Specific Antigen Inhalation in Equine Asthma

International Journal of Clinical and Experimental Medicine

Expression of MMP-9 and TIMP-1 in rat models of pressure ulcer and their significance

Antioxidants

Hydroxytyrosol Supplementation Modifies Plasma Levels of Tissue Inhibitor of Metallopeptidase 1 in Women with Breast Cancer Pubmed: 31514476

Journal of Obstetrics and Gynaecology Research

The levels of matrix metalloproteinase-9 and neutrophil gelatinase-associated lipocalin in different stages of endometriosis Pubmed: 31177884

Archives of Insect Biochemistry and Physiology

Evaluation of the anti-atherogenic potential of Egyptian artichoke leaf extract in hypercholesterolemic rats Pubmed: 31566004

Medicina

Proportion of the CD19-Positive and CD19-Negative Lymphocytes and Monocytes within the Peripheral Blood Mononuclear Cell Set Is Characteristic for Rheumatoid … Pubmed: 31554310

Oncology Research

MicroRNA-6884-5p regulates the proliferation, invasion and EMT of gastric cancer cells by directly targeting S100A16 Pubmed: 31796150

Open Medicine

Plasma matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1 as prognostic biomarkers in critically ill patients Pubmed: 32190734

Scientific Reports

Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis Pubmed: 31980722

JOURNAL OF THROMBOSIS AND THROMBOLYSIS

Extracellular matrix collagen biomarkers levels in patients with chronic thromboembolic pulmonary hypertension Pubmed: 33175289

Eur J Pharmacol

TLR3 inhibitor and tyrosine kinase inhibitor attenuate cigarette smoke/poly I: C-induced airway inflammation and remodeling by the EGFR/TLR3/MAPK … Pubmed: 33068589

Cancers

Multiple Myeloma-Derived Extracellular Vesicles Induce Osteoclastogenesis through the Activation of the XBP1/IRE1α Axis Pubmed: 32759820

Lab Invest

Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitis Pubmed: 32709888

International Journal of Environmental Research and Public Health

Biomarkers Facilitate the Assessment of Prognosis in Critically Ill Patients with Primary Brain Injury: A Cohort Study Pubmed: 32575870

J Cell Physiol

A novel role of VEPH1 in regulating AoSMC phenotypic switching Pubmed: 32342520

JAMA Surgery

Intraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairment Pubmed: 28593326

OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY

Intracranial Aneurysm Biomarker Candidates Identified by a Proteome-Wide Study Pubmed: 32525733

Advances in Animal and Veterinary Sciences

Clinicopathological studies and new markers for trypanosoma evansi in experimen-tally infected rats

International Journal of Pharmacology

Anti-arthritic Effects of Platelets Rich Plasma and Hyaluronic Acid on Adjuvant-induced Arthritis in Rats

Asian Journal of Pharmacy and Pharmacology

Evaluation of altered ground matrix and matrix metalloproeinase (MMP'S) in wound healing with Aloe vera extract

Veterinary Sciences: Research and Reviews

Investigation of matrix metalloproteinase-2 and matrix metalloproteinase-9 activity in some inflammatory conditions in Barki sheep


Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats

Клиническая медицина

ДИНАМИКА МАРКЕРА ФИБРОЗИРОВАНИЯ ММР-9 У БОЛЬНЫХ ЛИМФОМОЙ ХОДЖКИНА

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH

Krill oil and low-dose aspirin combination mitigates experimentally induced silicosis in rats: role of NF-¦ÊB/TGF-¦Â1/MMP-9 pathway 33398741

Oncol Lett

GATA3 is downregulated in HCC and accelerates HCC aggressiveness by transcriptionally inhibiting slug expression 33613720

CLINICAL SCIENCE

Berberine accelerated wound healing by restoring TrxR1/JNK in diabetes 33491733

Sci Rep

Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population 33850223

MOLECULAR VISION

Quantification of a panel for dry-eye protein biomarkers in tears: A comparative pilot study using standard ELISA and customized microarrays 34012227

Journal of Pregnancy

Matrix Metalloproteinases MMP-2 and MMP-9 Occupy a New Role in Severe Preeclampsia 33381317

Stem Cells International

The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin?¡­ 33381190

DOCSBAY

Intermittent Pacing Therapy Favorably Modulates Infarct Remodeling 28386775

Drug Chem Toxicol

Study of cardioprotective activity of the methanolic extract of the aerial parts of Bauhinia madagascariensis compared to Bauhinia purpurea against adrenaline … 34167392

JOURNAL OF FLUORESCENCE

Time-resolved Fluorescence Immunoassay (TRFIA) for the Simultaneous Detection of MMP-9 and Lp-PLA2 in Serum 34495467

CNS Neurosci Ther

Alterations in the gut microbiome with hemorrhagic transformation in experimental stroke 34591349


Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and EMT Process in Gastric Cancer 34650982

Disease Markers

Urinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study 34707724

Medicina (Kaunas)

Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study Pubmed:35743980

Biomolecules

Increase in Serum MMP-9 and TIMP-1 Concentrations during Alcohol Intoxication in Adolescents—A Preliminary Study Pubmed:35625637

Journal of Pharmaceutical Research International

Concomitant Administration of Rosuvastatin and Lefleunamide in Low doses Synergize Against Complete Freund's Adjuvant (CFA)-Induced Rheumatoid …


Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …

Turkish Journal of Biochemistry-Turk Biyokimya Dergisi

Downregulation of MMP-2 and MMP-9 genes in obesity patients and their relation with obesity-related phenotypes


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Applications (RESEARCH USE ONLY!)

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WB; IHC; ICC; IP.

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WB; IHC; ICC; IP.

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WB; IHC; ICC.

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WB; IHC; ICC; IF.

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Flow cytometry.

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WB; IHC; ICC; IP.

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WB

MAA553Hu23

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WB; IHC; ICC; IP.

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WB; IHC; ICC; IP.

MAA553Hu25

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WB; IHC; ICC; IP.

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WB; IHC; ICC; IP.

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WB; IHC; ICC; IP.

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WB; IHC; ICC; IP.

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Enzyme-linked immunosorbent assay for Antigen Detection.

SCA553Hu

CLIA Kit for Matrix Metalloproteinase 9 (MMP9)

Chemiluminescent immunoassay for Antigen Detection.

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Magnetic Luminex Assay Kit for Matrix Metalloproteinase 9 (MMP9) ,etc.

Magnetic Luminex Assay for Antigen Detection.

KSA553Hu01

ELISA Kit DIY Materials for Matrix Metalloproteinase 9 (MMP9)

Main materials for "Do It (ELISA Kit) Yourself".

 

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