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SKU:LAA437Hu81
FITC-Linked Polyclonal Antibody to Estrogen Receptor Beta (ERb)
FITC-Linked Polyclonal Antibody to Estrogen Receptor Beta (ERb)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAA437Hu81
Organism Species
Homo sapiens (Human).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
FITC
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IF.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against ERb. It has been selected for its ability to recognize ERb in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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The Journal of Steroid Biochemistry and Molecular Biology |
Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signalling Pubmed:24846829 |
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Biochem Biophys Res Commun |
Differential expression of estrogen receptor α and β isoforms in multiple and solitary leiomyomas PubMed: 26529545 |
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Mol Neurobiol |
Selective Aryl Hydrocarbon Receptor Modulator 3,3′-Diindolylmethane Impairs AhR and ARNT Signaling and Protects Mouse Neuronal Cells Against Hypoxia PubMed: 26476840 |
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J Steroid Biochem Mol Biol |
Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR31 signalling PubMed: 24846830 |
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Journal of Environmental Sciences |
Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts science:S1001074216300924 |
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Biol Open. |
Involvement of BDNF/TrkB and ERK/CREB axes in nitroglycerin-induced rat migraine and effects of estrogen on these signals in the migraine. pubmed:27875242 |
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Biomedicine & Pharmacotherapy |
Postmenopausal osteoporosis is associated with the regulation of SP, CGRP, VIP, and NPY Pubmed:29807224 |
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Journal of Applied Pharmaceutical Science |
Physical Signs of Illness, Liver Functions and Reproductive Parameters of Female Rats Supplemented with Melaleuca cajuputi Methanolic Extract Pbulication:325682424 |
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Carleton University Research Virtual Environment |
In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts |
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Life Sciences |
MiR-145 regulates steroidogenesis in mouse primary granulosa cells through targeting Crkl 34273377 |
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Cardiometry |
Modifying effect of obesity on the content of sex hormones and their receptors in endometrial adenocarcinoma and its surrounding tissue |
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