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SKU:LAA172Hu81

FITC-Linked Polyclonal Antibody to Platelet Factor 4 (PF4)

FITC-Linked Polyclonal Antibody to Platelet Factor 4 (PF4)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

LAA172Hu81

Organism Species

Homo sapiens (Human).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

FITC

Original Antibody

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IF.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against PF4. It has been selected for its ability to recognize PF4 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics

Propofol lowers serum PF4 level and partially corrects hypercoagulopathy in endotoxemic rats PubMed: 20601223

Basic Research in Cardiology

Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation PubMed: 22899170

PLoS One

Platelets Recognize Brain-Specific Glycolipid Structures, Respond to Neurovascular Damage and Promote Neuroinflammation PubMed: PMC3608633

Diving and Hyperbaric Medicine

Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompression Pubmed:25311322

Journal of Functional Foods

A Phellinus baumii–based supplement containing Salvia miltiorrhiza Bunge improves atherothrombotic profiles through endothelial nitric oxide synthase and cyclooxygenase pathwaysin vitro and in vivo science:S1756464616300676

Plos one

Aspirin, but Not Tirofiban Displays Protective Effects in Endotoxin Induced Lung Injury pubmed:27583400

international journal of molecular sciences

Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation. pubmed:28272338

Experimental and Therapeutic Medicine

Feasibility of improving platelet‑rich plasma therapy by using chitosan with high platelet activation ability pubmed:28450960

Reproductive Toxicology

Aspirin pre-treatment modulates ozone-induced fetal growth restriction and alterations in uterine blood flow in rats Pubmed: 30528429

INTERNATIONAL JOURNAL OF CARDIOLOGY

Short and long-term changes in platelet and inflammatory biomarkers after cryoballoon and radiofrequency ablation Pubmed: 30857843

Available at SSRN 3335365 

Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers

journal of biotechnology

Newly Identified HNP‐F from Human Neutrophil Peptide 1 Promotes Hemostasis Pubmed: 30927490

Data in Brief

Data for short and long-term prothrombotic biomarkers after cryoballoon and radiofrequency ablation

journal of thoracic and cardiovascular surgery

Evaluating Platelet Activation Related to the Degradation of Biomaterials Using Molecular Markers Pubmed: 32812629

Marine Drugs

The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in Rodents Pubmed: 31533230

Thrombosis Research

Feasibility study of use of rabbit blood to evaluate platelet activation by medical devices Pubmed: 31838449

Nat Commun

The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets 34099640

Vaccine

The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats Pubmed:35183388


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