Cloud-clone
SKU:LAB792Mu81
FITC-Linked Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8)
FITC-Linked Polyclonal Antibody to S100 Calcium Binding Protein A8 (S100A8)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAB792Mu81
Organism Species
Mus musculus (Mouse).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
FITC
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IF.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against S100A8. It has been selected for its ability to recognize S100A8 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Biochemical and Biophysical Research Communications |
High circulating levels of S100A8/A9 complex (calprotectin) in male Japanese with abdominal adiposity and dysregulated expression of S100A8 and S100A9 in adipose tissues of obese mice ScienceDirect: S0006291X1200352X |
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American Journal of Reproductive Immunology |
Association of Increased S100A8 Serum Protein with Early Pregnancy Loss Pubmed:25252120 |
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the faseb journal |
Soluble receptor for advanced glycation end products inhibits disease progression in autosomal dominant polycystic kidney disease by down-regulating cell proliferation PubMed: 25934702 |
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J Immunol |
S100A8 Production in CXCR2-Expressing CD11b+ Gr-1high Cells Aggravates Hepatitis in Mice Fed a High-Fat and High-Cholesterol Diet PubMed: 26608915 |
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PLoS One |
Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation Pubmed:26889680 |
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Journal of Alzheimer's Disease |
Proteomics Analysis of Blood Serums from Alzheimer's Disease Patients Using iTRAQ LabelingTechnology. pubmed:27911324 |
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Journal of Functional Foods |
Standardized ethanolic extract of the rhizome of Curcuma xanthorrhiza prevents murine ulcerative colitis by regulation of inflammation S1756464617300208 |
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International Journal of Molecular Medicine |
Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells pubmed:28498464 |
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International journal of molecular sciences |
Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds. pubmed:28672877 |
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Gastroenterology |
Tu1938-Micronutrients Assessment in Patients with Cirrhosis 10.1016:S0016-5085(18)33544-3 |
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Gastroenterology |
Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis 10.1016:S0016-5085(18)33544-5 |
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Journal of Biological Chemistry |
Signaling between pancreatic β-cells and macrophages via S100 calcium-binding protein A8 exacerbates β-cell apoptosis and islet inflammation Pubmed:29496993 |
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European review for medical and pharmacological sciences |
S100A8/A9 promotes MMP-9 expression in the fibroblasts from cardiac rupture after myocardial infarction by inducing macrophages secreting TNFα. Pubmed:29949169 |
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Foodborne Pathogens and Disease |
Synergistic effect of immunomodulatory S100A8/A9 and ciprofloxacin against Pseudomonas aeruginosa biofilm in a murine chronic wound model Pubmed: 31116394 |
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Kidney International Reports |
Systemic Inflammation Precedes Microalbuminuria in Diabetes |
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Cancer Management and Research |
iTRAQ-based quantitative protein expression profiling of biomarkers in childhood B-cell and T-cell acute lymphoblastic leukemia Pubmed: 31440093 |
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scandinavian journal of clinical & laboratory investigation |
Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study Pubmed: 31856598 |
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ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE |
Analysis of novel serum markers of fibrosis and angiogenesis in patients with alcoholic liver cirrhosis |
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MOLECULAR VISION |
Quantification of a panel for dry-eye protein biomarkers in tears: A comparative pilot study using standard ELISA and customized microarrays 34012227 |
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university of copenhagen |
Adjunctive S100A8/A9 Immunomodulation Hinders Cipro fl oxacin Resistance in |
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Main materials for "Do It (ELISA Kit) Yourself". |
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