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SKU:LAB091Mu81

FITC-Linked Polyclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL)

FITC-Linked Polyclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

LAB091Mu81

Organism Species

Mus musculus (Mouse).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

FITC

Original Antibody

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

Homo sapiens (Human), Rattus norvegicus (Rat)

Applications

WB; IHC; ICC; IF.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ALPL. It has been selected for its ability to recognize ALPL in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Journal of Materials Science: Materials in Medicine

Osteopenic bone cell response to strontium-substituted hydroxyapatite SpringerLink: n8233337w171v451

Osteoporosis International

Dextromethorphan inhibits osteoclast differentiation by suppressing RANKL-induced nuclear factor-κB activation PubMed: 23400250

Journal of Applied Toxicology

Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice Wiley: Source

Acta Pharmacologica Sinica

The antidepressant bupropion exerts alleviating properties in an ovariectomized osteoporotic rat model Pubmed:25544359

Biomedical Materials

Enhanced differentiation of osteoblastic cells on novel chitosan/β-1, 3-glucan/bioceramic scaffolds for bone tissue regeneration Pubmed:25586067

Pharmacology

Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy PubMed: 25871861

J Biomed Mater Res A.

New method for the fabrication of highly osteoconductive β-1,3-glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological characterization. Pubmed:27239050

J Bone Miner Metab

MiR-142-5p promotes bone repair by maintaining osteoblast activity Pubmed:27085967

Journal of Biomedical Materials Research

New method for the fabrication of highly osteoconductive β‐1, 3‐glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological … doi:10.1002

Journal of Bone and Mineral Metabolism

MiR‑142‑5p promotes bone repair by maintaining osteoblast activity pubmed:27085967

Scientific Reports

Hydrolysis of Extracellular Pyrophosphate increases in post-hemodialysis plasma Pubmed:30038263

International Journal of Molecular Sciences 

Suppression Effect of Astaxanthin on Osteoclast Formation In Vitro and Bone Loss In Vivo Pubmed:29562730

Pharmacology

Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats Pubmed:29898457

Experimental and Therapeutic Medicine

Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized mice 10.3892:etm.2018.6353

American Journal of Translational Research

Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9


The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro

International Journal of Biomaterials

Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect 34104195


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