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SKU:LAA684Mu81

FITC-Linked Polyclonal Antibody to Mucin 5 Subtype B (MUC5B)

FITC-Linked Polyclonal Antibody to Mucin 5 Subtype B (MUC5B)

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UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL
;Immunohistochemistry: 5-20µg/mL
;Immunocytochemistry: 5-20µg/mL
;Optimal working dilutions must be determined by end user.

Product No.

LAA684Mu81

Organism Species

Mus musculus (Mouse).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

FITC

Original Antibody

PAA684Mu01-Polyclonal Antibody to Mucin 5 Subtype B (MUC5B)

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

Applications: WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against MUC5B. It has been selected for its ability to recognize MUC5B in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

The Journal of Biological Chemistry

The Extracellular Signal-regulated Kinase Mitogen-activated Protein Kinase/Ribosomal S6 Protein Kinase 1 Cascade Phosphorylates cAMP Response Element-binding Protein to Induce MUC5B Gene Expression via D-Prostanoid Receptor Signaling Jcb: 34199

The Journal of Biological Chemistry

The ERK MAPK/RSK1 cascade phosphorylates CREB to induce MUC5B gene expression via DP1 receptor signaling Jcb: 247684

Respiratory Researh

Pyocyanin-induced mucin production is associated with redox modification of FOXA2 PubMed: PMC3765780

Respirology

Secreted mucins and airway bacterial colonization in non-CF bronchiectasis PubMed: 26172851

American Thoracic Society International Conference Abstracts

Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsiveness doi:10.1164

Ann Am Thorac Soc

Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization doi:10.1513


Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой 10.20538/1682-0363-2017-2-146-158

Allergy

Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polyps Pubmed:29802623

European Journal of Pharmacology 

Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling Pubmed:29382534

Japan Society of Immunology & Allergology in Otolaryngology

インフルエンザ菌Phosphorylcholine の表出と中耳ムチンMUC5AC 及びMUC5B 産生への影響 10.5648:jjiao.36.7

Journal of Investigative Medicine

The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease Pubmed: 30573494

Mucosal Immunology

ASC and NLRP3 maintain innate immune homeostasis in the airway through an inflammasome-independent mechanism Pubmed: 31278375


Catalog No.

Related products for research use of Mus musculus (Mouse) Organism species

Applications (RESEARCH USE ONLY!)

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LAA684Mu81

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Applications: WB; IHC; ICC; IP.

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WB; IHC; ICC.

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Enzyme-linked immunosorbent assay for Antigen Detection.

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Magnetic Luminex Assay for Antigen Detection.

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ELISA Kit DIY Materials for Mucin 5 Subtype B (MUC5B)

Main materials for "Do It (ELISA Kit) Yourself".

 

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