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SKU:MAB355Hu21

Monoclonal Antibody to 5-Lipoxygenase (5-LO)

Monoclonal Antibody to 5-Lipoxygenase (5-LO)

Regular price $352.00 USD
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UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL
;Immunohistochemistry: 5-20µg/mL
;Immunocytochemistry: 5-20µg/mL
;Optimal working dilutions must be determined by end user.

Product No.

MAB355Hu21

Organism Species

Homo sapiens (Human).

Source

Monoclonal antibody preparation

Host

Mouse

Potency (Clone Number)

D10

Ig Isotype

IgG2b Kappa

Purification

Protein A + Protein G affinity chromatography

Label

None

Immunogen

RPB355Hu01-Recombinant 5-Lipoxygenase (5-LO)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

1mg/mL

Organism Species More

Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Applications

WB

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a mouse monoclonal antibody raised against 5-LO. It has been selected for its ability to recognize 5-LO in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Magazine Citations
Prostaglandins & Other Lipid Mediators Modulating effect of inositol hexaphosphate on arachidonic acid-dependent pathways in colon cancer cells. pubmed:28797636
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB355Hu02 Recombinant 5-Lipoxygenase (5-LO) Positive Control; Immunogen; SDS-PAGE; WB.
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