Cloud-clone
SKU:PAA998Ra01
Polyclonal Antibody to Glypican 4 (GPC4)
Polyclonal Antibody to Glypican 4 (GPC4)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAA998Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPA998Ra01-Recombinant Glypican 4 (GPC4)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
0.47mg/ml
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against GPC4. It has been selected for its ability to recognize GPC4 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| PLoS One. | Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation Pubmed:Pmc4072672 |
| Aspects of fluid therapy in the critically ill. Experimental and clinical studies on fluid therapy in the inflammatory conditions. 250690_2_G5_Svajunas_S.pdf | |
| Intensive Care Medicine Experimental | Elevated plasma glypicans are associated with organ failure in patients with infection Pubmed: 30618011 |
| Crit Care | Albumin infusion rate and plasma volume expansion: a randomized clinical trial in postoperative patients after major surgery Pubmed: 31138247 |
| J Endocrinol Invest | Serum glypican4 and glycosylphosphatidylinositol-specific phospholipase D levels are associated with adipose tissue insulin resistance in obese subjects with … Pubmed: 32816247 |
| Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study | |
| Sci Rep | Glypican-4 in pregnancy and its relation to glucose metabolism, insulin resistance and gestational diabetes mellitus status 34903856 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA998Ra01 | Recombinant Glypican 4 (GPC4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA998Ra01 | Polyclonal Antibody to Glypican 4 (GPC4) | WB; IHC; ICC; IP. |
| SEA998Ra | ELISA Kit for Glypican 4 (GPC4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA998Ra | Magnetic Luminex Assay Kit for Glypican 4 (GPC4) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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