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SKU:MAA489Ra21
Monoclonal Antibody to Cytokeratin 4 (CK4)
Monoclonal Antibody to Cytokeratin 4 (CK4)
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Western blotting: 0.5-5ug/ml
;Immunocytochemistry in formalin fixed cells: 5-30ug/ml
;Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
;Immunohistochemistry in paraffin section: 5-30ug/ml
;Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
;Optimal working dilutions must be determined by end user.
Product No.
MAA489Ra21
Organism Species
Rattus norvegicus (Rat).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
1:270000 (1#)
Ig Isotype
IgG1 Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPA489Ra01-Recombinant Cytokeratin 4 (CK4)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
1mg/mL
Organism Species More
Mus musculus (Mouse)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against CK4. It has been selected for its ability to recognize CK4 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Molecular Medicine Reports | Placental protein 14 as a potential biomarker for diagnosis of preterm premature rupture of membranes Pubmed:29749501 |
| FEBS Open Bio | Development of a novel murine heart failure model overexpressing human renin and angiotensinogen Pubmed: 32056390 |
| J Clin Periodontol | Discovery, validation, and diagnostic ability of multiple protein-based biomarkers in saliva and gingival crevicular fluid to distinguish between health and periodontal … Pubmed:35451104 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA489Ra01 | Recombinant Cytokeratin 4 (CK4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA489Ra01 | Polyclonal Antibody to Cytokeratin 4 (CK4) | WB; IHC; ICC; IP. |
| LAA489Ra71 | Biotin-Linked Polyclonal Antibody to Cytokeratin 4 (CK4) | WB; IHC; ICC. |
| MAA489Ra21 | Monoclonal Antibody to Cytokeratin 4 (CK4) | WB; IHC; ICC; IP. |
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