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SKU:PAA285Mi01
Polyclonal Antibody to Histone H3 (H3)
Polyclonal Antibody to Histone H3 (H3)
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Western blotting: 0.5-2µg/mL;
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.
Product No.
PAA285Mi01
Organism Species
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Rhesus monkey (Simian), Canis familiaris; Canine (Dog), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse), Chicken (Gallus).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPA285Mi01-Recombinant Histone H3 (H3)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
0.5mg/ml
Organism Species More
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Rhesus monkey (Simian), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse), Chicken (Gallus)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against H3. It has been selected for its ability to recognize H3 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Biomed Res Int | A Novel Anti-Histone H1 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome PubMed: 25649890 |
| Cell Death & Disease | Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death Pubmed:35732625 |
| Catalog No. | Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Rhesus monkey (Simian), Canis familiaris; Canine (Dog), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse), Chicken (Gallus) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA285Mi01 | Recombinant Histone H3 (H3) | Positive Control; Immunogen; SDS-PAGE; WB. |
| CAA285Mi01 | Anti-Histone H3 (H3) Polyclonal Antibody | Loading Control of WB. |
| PAA285Mi01 | Polyclonal Antibody to Histone H3 (H3) | WB; IHC; ICC; IP. |
| MAA285Mi21 | Monoclonal Antibody to Histone H3 (H3) | WB; IHC; ICC; IP. |
| MAA285Mi22 | Monoclonal Antibody to Histone H3 (H3) | WB; IHC; ICC; IP. |
| MAA285Mi23 | Monoclonal Antibody to Histone H3 (H3) | WB; IHC; ICC; IP. |
| MAA285Mi24 | Monoclonal Antibody to Histone H3 (H3) | WB; IHC; ICC; IP. |
| SEA285Mi | ELISA Kit for Histone H3 (H3) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA285Mi | Magnetic Luminex Assay Kit for Histone H3 (H3) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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