Cloud-clone
SKU:PAB342Mi01
Polyclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2)
Polyclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2)
Couldn't load pickup availability
Western blotting: 0.5-2µg/mL;
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.
Product No.
PAB342Mi01
Organism Species
Mus musculus (Mouse).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPB342Mi01-Recombinant Actin Alpha 2, Smooth Muscle (ACTa2)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
0.5mg/ml
Organism Species More
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Oryctolagus cuniculus (Rabbit), Rhesus monkey (Simian), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse), Chicken (Gallus)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
10µg 50µg 200µg 1mg 5mg
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against ACTa2. It has been selected for its ability to recognize ACTa2 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-5µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Cell Mol Immunol | Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica Pubmed:27157496 |
| NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY | Effects of repeated increasing doses of cisplatin as models of acute kidney injury and chronic kidney disease in rats Pubmed: 32936352 |
| Brazilian Journal of Pharmaceutical Sciences | Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm |
| Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells | |
| Parasit Vectors | Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate … 34857049 |
| Clinical and Translational Medicine | NOX5 mediates the crosstalk between tumor cells and cancer‐associated fibroblasts via regulating cytokine network 34459125 |
Share
