Cloud-clone
SKU:PAL920Ga01
Polyclonal Antibody to Semaphorin 3E (SEMA3E)
Polyclonal Antibody to Semaphorin 3E (SEMA3E)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAL920Ga01
Organism Species
Chicken (Gallus).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against SEMA3E. It has been selected for its ability to recognize SEMA3E in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Ophthalmology | Aqueous Levels of Angiopoietin-like 4 and Semaphorin 3E Correlate with Nonperfusion Area and Macular Volume in Diabetic Retinopathy PubMed: 25687026 |
| annals of clinical and laboratory science | Association of Increased Serum Sema3E with TRIB3 Q84R Polymorphism and Carotid Atherosclerosis in Metabolic Syndrome. pubmed:28249916 |
| INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE | Spatial association of SEMA3C with nerve endings/terminal Schwann cells in hair follicle isthmus region Pubmed: 32954569 |
| ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY | The effect of nutrition and exercise training on irisin and semaphorin-3E levels in obese patients Pubmed: 32569480 |
| EMBO JOURNAL | RhoJ integrates attractive and repulsive cues in directional migration of endothelial cells Pubmed: 32347571 |
| Catalog No. | Related products for research use of Chicken (Gallus) Organism species | Applications (RESEARCH USE ONLY!) |
| RPL920Ga01 | Recombinant Semaphorin 3E (SEMA3E) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAL920Ga01 | Polyclonal Antibody to Semaphorin 3E (SEMA3E) | WB; IHC; ICC; IP. |
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