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SKU:MAF880Hu21
Monoclonal Antibody to Secreted Frizzled Related Protein 1 (SFRP1)
Monoclonal Antibody to Secreted Frizzled Related Protein 1 (SFRP1)
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Western blotting: 0.5-2µg/mL;
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.
Product No.
MAF880Hu21
Organism Species
Homo sapiens (Human).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
C5
Ig Isotype
IgG1 Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPF880Hu01-Recombinant Secreted Frizzled Related Protein 1 (SFRP1)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/mL
Organism Species More
n/a
Applications
WB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against SFRP1. It has been selected for its ability to recognize SFRP1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| BMC Musculoskeletal Disorders | Biomarkers and cytokines of bone turnover: extensive evaluation in a cohort of patients with ankylosing spondylitis PubMed: 23025387 |
| Exp Cell Res | Wnt Inhibition is Dysregulated in Gliomas and its Re-Establishment Inhibits Proliferation and Tumor Sphere Formation PubMed: 26712519 |
| BMC Musculoskeletal Disorders | Correlation of blood bone turnover biomarkers and Wnt signaling antagonists with AS, DISH,OPLL, and OYL. pubmed:28153008 |
| PloS one | Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial. pubmed:28178355 |
| PLoS One | Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial 10.1371 |
| Journal of Experimental & Clinical Cancer Research | PHF21B overexpression promotes cancer stem cell-like traits in prostate cancer cells by activating the Wnt/β-catenin signaling pathway pubmed:28645312 |
| JBMR Plus | Low serum levels of DKK2 predict incident low‐impact fracture in older women Doi: 10.1002/jbm4.10179 |
| Nature Communications | Immunological history governs human stem cell memory CD4 heterogeneity via the Wnt signaling pathway Pubmed: 32041953 |
| acta pharmacologica sinica | sFRP1 protects H9c2 cardiac myoblasts from doxorubicin-induced apoptosis by inhibiting the Wnt/PCP-JNK pathway Pubmed: 32238888 |
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