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SKU:MAH337Hu21
Monoclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a)
Monoclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a)
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Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
Product No.
MAH337Hu21
Organism Species
Homo sapiens (Human).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
n/a
Ig Isotype
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
1mg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against PPARgC1a. It has been selected for its ability to recognize PPARgC1a in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Coronary Artery Disease | Impaired myocardium energetics associated with the risk for new-onset atrial fibrillation after isolated coronary artery bypass graft surgery Pubmed: 24463787 |
| Molecular and cellular biochemistry | Skeletal muscle mitochondrial dysfunction precedes right ventricular impairment in experimental pulmonary hypertension Pubmed: 23099843 |
| Diabetes. | Inorganic nitrate promotes the browning of white adipose tissue through the nitrate-nitrite-nitric oxide pathway Pubmed:25249574 |
| Psychoneuroendocrinology | Maternal stress predicts altered biogenesis and the profile of mitochondrial proteins in the frontal cortex and hippocampus of adult offspring rats PubMed: 26143539 |
| Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry | The level of circulating PGC1α in cardiovascular diseases Article: 10.1134 |
| Neuroscience Letters | Unlike PPARgamma, neither other PPARs nor PGC-1alpha is elevated in the cerebrospinal fluid of patients with multiple sclerosis pubmed:28483651 |
| The American Journal of the Medical Sciences | Serum peroxisome proliferator-activated receptor gamma coactivator-1α related to myocardial energy expenditure in patients with chronic heart failure Doi: 10.1016/j.amjms.2018.12.002 |
| Brain Research | The effect of antecedent-conditioning high-intensity interval training on BDNF regulation through PGC-1α pathway following cerebral ischemia Pubmed: 31866362 |
| Food & Function | Ubiquinol supplementation modulates energy metabolism and bone turnover during high intensity exercise Pubmed: 32797125 |
| Brown and beige adipose tissue regulate systemic metabolism to resist diet-induced obesity through metabolite signals in an inter-organ signaling axis | |
| CLINICAL NUTRITION | Long lasting protective EFFECTS of early L-ARGININE TREAMENT on ENDOTHELIUM in an in Vitro STUDY 33743287 |
| Nature Communications | Brown and beige adipose tissue regulate systemic metabolism through a metabolite interorgan signaling axis 33772024 |
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