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SKU:MAH757Ra21
Monoclonal Antibody to Klotho (KL)
Monoclonal Antibody to Klotho (KL)
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$400.00 USD
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$400.00 USD
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Western blotting: 0.5-2µg/mL;1:500-2000
;Immunohistochemistry: 5-20µg/mL;1:50-200
;Immunocytochemistry: 5-20µg/mL;1:50-200
;Optimal working dilutions must be determined by end user.
Product No.
MAH757Ra21
Organism Species
Rattus norvegicus (Rat).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
D1
Ig Isotype
IgG2b Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPH757Ra01-Recombinant Klotho (KL)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against KL. It has been selected for its ability to recognize KL in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Life Sciences | High phosphate feeding induced arterial medial calcification in uremic rats: Roles of Lanthanum carbonate on protecting vasculature Pubmed: 24012609 |
| EXPERIMENTAL & CLINICAL CARDIOLOGY | Sick Sinus Syndrome May Attribute to the Decrease of ?±-klotho Protein in Mice Com:Source |
| Int J Clin Exp Pathol.? | α-Klotho is an acute phase protein and altered by restraint stress in mice Pubmed:Pmc4203207 |
| Annales de Biologie Clinique | Les biomarqueurs des calcifications vasculaires PubMed: 26069069 |
| Mol Med Rep | Effect of secondary hyperparathyroidism serum on endothelial cells and intervention with Klotho PubMed: 25873020 |
| Calcif Tissue Int | Human Preosteoblastic Cell Culture from a Patient with Severe Tumoral Calcinosis-Hyperphosphatemia Due to a NewGALNT3 PubMed: 25899975 |
| 中华内分泌代谢杂志 | 抗衰老蛋白Klotho 阻断高糖培养的大鼠肾小球系膜细胞TLR4/NF-κB p65/NGAL 通路的激活及其作用机制 Cn: Article |
| Mol Cell Endocrinol | The function of miR-199a-5p/Klotho regulating TLR4/NF-κB p65/NGAL pathways in rat mesangial cells cultured with high glucose and the mechanism PubMed: 26419931 |
| Arch Med Sci | Soluble Klotho protein as a novel serum biomarker in patients with acromegaly pmc:PMC4754356 |
| Am J Nephrol | Klotho Deficiency Aggravates Tacrolimus-Induced Renal Injury via the Phosphatidylinositol 3-Kinase-Akt-Forkhead Box Protein O Pathway Pubmed:27174564 |
| Sci Rep. | Klotho preservation via histone deacetylase inhibition attenuates chronic kidney disease-associated bone injury in mice. pubmed:28387374 |
| Nephrology | Is there a role for newer biomarkers in chronic kidney disease-mineral and bone disorder management? pubmed:28429560 |
| Journal of Integrative Nephrology & Andrology | Associations between serum fibroblast growth factor 23 level and intradialytic hypotension in hemodialysis patients issn:2394-2916 |
| International Journal of Clinical and Experimental Pathology | Protective effect of restructuring klotho protein combined with trimetazidine on myocardial injury in mice ISSN:1936-2625 |
| Journal of veterinary medical science | Changes of Klotho protein and Klotho mRNA expression in a hydroxy-L-proline induced hyperoxaluric rat model pubmed:28943597 |
| Cellular Physiology and Biochemistry | Klotho Reduces Necroptosis by Targeting Oxidative Stress Involved in Renal Ischemic-Reperfusion Injury Pubmed:29550818 |
| American Journal of Translational Research | Advanced maternal age impairs spatial learning capacity in young adult mouse offspring Pubmed:29636887 |
| Journal of Diabetes and its Complications | Clinical implication of alterations in serum Klotho levels in patients with type 2 diabetes mellitus and its associated complications Pubmed:30042059 |
| Medical Science Monitor | Klotho Inhibits Proliferation and Migration of Angiotensin II-Induced Vascular Smooth Muscle Cells (VSMCs) by Modulating NF-κB p65, Akt, and Extracellular Signal … Pubmed:30004089 |
| Nephrology Dialysis Transplantation | High-serum phosphate and parathyroid hormone distinctly regulate bone loss and vascular calcification in experimental chronic kidney disease Pubmed: 30189026 |
| BMC Nephrology | The role of klotho in chronic kidney disease Pubmed: 30348110 |
| Nature Communications | Age-related declines in α-Klotho drive progenitor cell mitochondrial dysfunction and impaired muscle regeneration Pubmed: 30451844 |
| Kidney International | Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndrome Pubmed: 30770217 |
| Expert Opinion on Therapeutic Targets | The use of LP533401 as a therapeutic option for renal osteodystrophy affects, renal calcium handling, vitamin D metabolism and bone health in uremic rats Pubmed: 30801205 |
| Mol Immunol | CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection Pubmed: 33223223 |
| Journal of Clinical Hypertension | Effects of salt intervention on serum levels of Klotho influenced by salt sensitivity Pubmed: 33164306 |
| CURRENT EYE RESEARCH | Expression of α-Klotho is downregulated and associated with oxidative stress in the lens in streptozotocin-induced diabetic rats Pubmed: 32744464 |
| international immunopharmacology | Klotho ameliorates the onset and progression of cataract via suppressing oxidative stress and inflammation in the lens in streptozotocin-induced diabetic rats Pubmed: 32438076 |
| Purinergic Signalling | P2X7 siRNA targeted to the kidneys increases klotho and delays the progression of experimental diabetic nephropathy. Pubmed: 32377919 |
| Oxidative Medicine and Cellular Longevity | Ameliorating Effect of Klotho Protein on Rat Heart during I/R Injury Pubmed: 33123314 |
| NEUROSCIENCE | Increased Plasma Level of Longevity Protein Klotho as a Potential Indicator of Cognitive Function Preservation in Patients With Schizophrenia Pubmed: 32612508 |
| Pharmacological Reports | ¦ÁKlotho protein has therapeutic activity in contrast-induced acute kidney injury by limiting NLRP3 inflammasome-mediated pyroptosis and promoting autophagy 33675964 |
| J Mol Neurosci | Investigation of the Role of Induced Overexpression of the Isolated Secreted Klotho on the A-172 Human Glioblastoma Cells Pubmed:35112226 |
| Reproductive Biology and Endocrinology | Expression of Klotho in Follicles of Patients With Polycystic Ovary Syndrome and Its Clinical Significance |
| Journal of Ovarian Research | Klotho is a prognostic indicator of oocytes qualities in polycystic ovary syndrome |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| APH757Ra01 | Active Klotho (KL) | Cell culture; Activity Assays. |
| RPH757Ra02 | Recombinant Klotho (KL) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPH757Ra01 | Recombinant Klotho (KL) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAH757Ra02 | Polyclonal Antibody to Klotho (KL) | WB; IHC; ICC; IP. |
| PAH757Ra01 | Polyclonal Antibody to Klotho (KL) | WB; IHC; ICC; IP. |
| MAH757Ra21 | Monoclonal Antibody to Klotho (KL) | WB; IHC; ICC; IP. |
| SEH757Ra | ELISA Kit for Klotho (KL) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMH757Ra | Magnetic Luminex Assay Kit for Klotho (KL) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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