Cloud-clone
SKU:MAK205Ra21
Monoclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5)
Monoclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5)
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Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
Product No.
MAK205Ra21
Organism Species
Rattus norvegicus (Rat).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
n/a
Ig Isotype
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
1mg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against ADAMTS5. It has been selected for its ability to recognize ADAMTS5 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source |
| Osteoarthritis and Cartilage | Lack of anti-inflammatory and anti-catabolic effects on basal inflamed osteoarthritic chondrocytes or synoviocytes by adipose stem cell-conditioned medium PubMed: 26521751 |
| Journal of Cellular and Molecular Medicine | VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblasts Pubmed:26818776 |
| Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748 |
| The American journal of pathology | Healthy and Osteoarthritic Synovial Fibroblasts Produce a Disintegrin and Metalloproteinase with Thrombospondin Motifs 4, 5, 7, and 12: Induction by IL-1β and Fibronectin and Contribution to Cartilage Damage. pubmed:27449198 |
| PLoS One. | Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders. pubmed:28207788 |
| PLoS One | Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders 10.1371:journal.pone.0172181 |
| International Wound Journal | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases pubmed:26991748 |
| BMC musculoskeletal disorders | Hemoglobin stimulates the expression of ADAMTS-5 and ADAMTS-9 by synovial cells: a possible cause of articular cartilage damage after intra-articular hemorrhage. pubmed:29137610 |
| Journal of Clinical Medicine | The Effects of TNF-α Inhibition on the Metabolism of Cartilage: Relationship between KS, HA, HAPLN1 and ADAMTS4, ADAMTS5, TOS and TGF-β1 Plasma … Pubmed:35407621 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPK205Ra01 | Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAK205Ra01 | Polyclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) | WB; IHC; ICC; IP. |
| MAK205Ra21 | Monoclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) | WB; IHC; ICC; IP. |
| SEK205Ra | ELISA Kit for A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMK205Ra | Magnetic Luminex Assay Kit for A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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