Cloud-clone
SKU:PAB972Bo01
Polyclonal Antibody to Pigment Epithelium Derived Factor (PEDF)
Polyclonal Antibody to Pigment Epithelium Derived Factor (PEDF)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAB972Bo01
Organism Species
Bos taurus; Bovine (Cattle).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against PEDF. It has been selected for its ability to recognize PEDF in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Molecular Therapy | A Therapeutic Strategy for Choroidal Neovascularization Based on Recruitment of Mesenchymal Stem Cells to the Sites of Lesions Nature: 2010144 |
| American Journal of Nephrology | Urinary Pigment Epithelium-Derived Factor as a Marker of Diabetic Nephropathy Karger: 314326 |
| 34 | Increased expression of pigment epithelium-derived factor in aged mesenchymal stem cells impairs their therapeutic efficacy for attenuating myocardial infarction injury OxfordJournals: source |
| Heart and Education in Heart | The change of serum level of pigment epithelium-derived factor in coronary heart disease Bmj: Source |
| Mediators of Inflammation | Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation Pubmed: 24288442 |
| Cardiology. | The association study of plasma levels of pigment epithelium-derived factor with acute coronary syndrome in the chinese han population. Pubmed: 24192856 |
| Endocrinology | Rosiglitazone Inhibits Expression and Secretion of PEDF in Adipose Tissue and Liver of Male SD Rats Via a PPAR-γ Independent Mechanism Endocrine: Source |
| Clinical Science | Pigment epithelium-derived factor regulates microvascular permeability through adipose triglyceride lipase in sepsis PubMed: 25700221 |
| Molecular Sciences | Pigment Epithelium-Derived Factor (PEDF) Protects Osteoblastic Cell Line from Glucocorticoid-Induced Apoptosis via PEDF-R Pubmed:27187377 |
| Investigative ophthalmoloey & visual science | The Ingenious Interactions Between Macrophages and Functionally Plastic Retinal Pigment Epithelium Cells articleid:2582822 |
| Scientific reports | VEGF-B promotes recovery of corneal innervations and trophic functions in diabetic mice pubmed:28091556 |
| 国际眼科杂志 | 色素上皮衍生因子修饰的人脐带间充质干细胞构建方法 201707007 |
| Investigative Ophthalmology & Visual Science | PEDF Reduces the Severity of Herpetic Simplex Keratitis in Mice Pubmed:30025136 |
| International Eye Science | 高海拔地区新生血管性青光眼不同治疗方式的疗效及对房水中PEDF, VEGF 水平的影响 2018/2/201802020.pdf |
| Domestic Animal Endocrinology | Lactation-related changes in tissue expression of PEDF in dairy cows Pubmed:29758402 |
| Medical Science Monitor | Correlation Between Pigment Epithelium-Derived Factor (PEDF) level and Degree of Coronary Angiography and Severity of Coronary Artery Disease in a … Pubmed:29574467 |
| Chemosphere | Role of pigment epithelium-derived factor (PEDF) on arsenic-induced neuronal apoptosis Doi: 10.1016/j.chemosphere.2018.10.100 |
| Biological Trace Element Research | Role of Pigment Epithelium-Derived Factor in Arsenic-Induced Vascular Endothelial Dysfunction in a Rat Model Pubmed: 30392020 |
| International Urology and Nephrology | Increased levels of serum pigment epithelium-derived factor aggravate proteinuria via induction of podocyte actin rearrangement Pubmed: 30536192 |
| International Journal of Ophthalmology | A novel xeno-free culture system for human retinal pigment epithelium cells Pubmed: 31024807 |
| Journal of the American Heart Association | Pigment Epithelial‐Derived Factor Deficiency Accelerates Atherosclerosis Development via Promoting Endothelial Fatty Acid Uptake in Mice With Hyperlipidemia Pubmed: 31711388 |
| environmental research | Pigment epithelium-derived factor (PEDF) ameliorates arsenic-induced vascular endothelial dysfunction in rats and toxicity in endothelial EA. hy926 cells Pubmed: 32315827 |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells 33498962 |
| Hum Cell | Long non-coding RNA Rian promotes the expression of tight junction proteins in endothelial cells by regulating perivascular-resident macrophage-like melanocytes ¡ 33768511 |
| Catalog No. | Related products for research use of Bos taurus; Bovine (Cattle) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB972Bo01 | Recombinant Pigment Epithelium Derived Factor (PEDF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB972Bo01 | Polyclonal Antibody to Pigment Epithelium Derived Factor (PEDF) | WB; IHC; ICC; IP. |
| MAB972Bo21 | Monoclonal Antibody to Pigment Epithelium Derived Factor (PEDF) | WB; IHC; ICC; IP. |
| SEB972Bo | ELISA Kit for Pigment Epithelium Derived Factor (PEDF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB972Bo | Magnetic Luminex Assay Kit for Pigment Epithelium Derived Factor (PEDF) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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