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SKU:PAC047Mu02

Polyclonal Antibody to Ki-67 Protein (Ki-67)

Polyclonal Antibody to Ki-67 Protein (Ki-67)

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UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL;
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.

Product No.

PAC047Mu02

Organism Species

Mus musculus (Mouse).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPC047Mu02-Recombinant Ki-67 Protein (Ki-67)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

1mg/ml

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against Ki-67. It has been selected for its ability to recognize Ki-67 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
BMC Research Notes Expressions and clinical significances of c-MET, p-MET and E2f-1 in human gastric carcinoma Pubmed: 24393368
BMC Research Notes Expressions and clinical significances of c-MET, p-MET and E2f-2 in human gastric carcinoma Pubmed:24393368
Chemistry & Biodiversity Chemical composition of aqueous‐ethanol extract of leaves and its effect on representation of caspase 8, caspase 3 and the proliferation marker Ki67 in intrinsic … Pubmed:29874411
Predictive value of the proliferation marker Ki-67 in patients with acute leukemia undergoing hematopoietic stem cell transplantation
Carbohydrate Polymers A novel exopolysaccharide from marine bacterium Pantoea sp. YU16-S3 accelerates cutaneous wound healing through Wnt/β-catenin pathway Pubmed: 32299547
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