Cloud-clone
SKU:PAC534Ra01
Polyclonal Antibody to Histidine Rich Glycoprotein (HRG)
Polyclonal Antibody to Histidine Rich Glycoprotein (HRG)
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Western blotting: 0.5-2µg/mL;1:150-600
;Immunohistochemistry: 5-20µg/mL;1:15-60
;Immunocytochemistry: 5-20µg/mL;1:15-60
;Optimal working dilutions must be determined by end user.
Product No.
PAC534Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPC534Ra01-Recombinant Histidine Rich Glycoprotein (HRG)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against HRG. It has been selected for its ability to recognize HRG in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| J. Proteome Res. | Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC–MS/MS ACS: pr300330z |
| Medical Oncology | Evaluation of histidine-rich glycoprotein tissue RNA and serum protein as novel markers for breast cancer Springer: Source |
| Int J Cancer | The nuclear bile acid receptor FXR controls the liver derived tumor suppressor histidine‐rich glycoprotein PubMed: 25363753 |
| Nucleic Acid Therapeutics | Pharmacokinetic and Pharmacodynamic Investigations of ION-353382, a Model Antisense Oligonucleotide: Using Alpha-2-Macroglobulin and Murinoglobulin Double-Knockout Mice Pubmed:27031383 |
| United States Patent Application | Compositions and Methods for Diagnosing Lung Cancer y2016:0077095.html |
| Hypertension Research | Combination of serum histidine-rich glycoprotein and uterine artery Doppler to predict preeclampsia Pubmed:29416108 |
| journal of histochemistry & cytochemistry | Ultrastructural Localization of Histidine-rich Glycoprotein in Skeletal Muscle Fibers: Colocalization With AMP Deaminase Pubmed: 31880188 |
| DOKLADY BIOCHEMISTRY AND BIOPHYSICS | Personalized Approach to Determination of Histidine-Rich Glycoprotein and E-Cadherin in Supernatants of Immunocompetent Blood Cells and Breast Biopsy … Pubmed: 32342301 |
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