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SKU:PAE132Bo01

Polyclonal Antibody to Defensin Beta 103A (DEFb103A)

Polyclonal Antibody to Defensin Beta 103A (DEFb103A)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

PAE132Bo01

Organism Species

Bos taurus; Bovine (Cattle).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against DEFb103A. It has been selected for its ability to recognize DEFb103A in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Plos Pathogens HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in?Pseudomonas aeruginosa?Keratitis PubMed: PMC3715414
Scientific Reports Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique Pubmed:29374283
PeerJ Antibacterial activity of human defensins against Staphylococcus aureus and Escherichia coli 33304659
Animals Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport 33375218
Sci Rep Human beta defensin levels and vaginal microbiome composition in post-menopausal women diagnosed with lichen sclerosus 34362937
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
RPE132Bo01 Recombinant Defensin Beta 103A (DEFb103A) Positive Control; Immunogen; SDS-PAGE; WB.
PAE132Bo01 Polyclonal Antibody to Defensin Beta 103A (DEFb103A) WB; IHC; ICC; IP.
MAE132Bo21 Monoclonal Antibody to Defensin Beta 103A (DEFb103A) WB; IHC; ICC; IP.
SEE132Bo ELISA Kit for Defensin Beta 103A (DEFb103A) Enzyme-linked immunosorbent assay for Antigen Detection.
LME132Bo Magnetic Luminex Assay Kit for Defensin Beta 103A (DEFb103A) ,etc. Magnetic Luminex Assay for Antigen Detection.
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