Cloud-clone
SKU:PAE640Hu01
Polyclonal Antibody to Receptor Interacting Serine Threonine Kinase 1 (RIPK1)
Polyclonal Antibody to Receptor Interacting Serine Threonine Kinase 1 (RIPK1)
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Western blotting: 0.5-2µg/mL;1:350-1400
Immunohistochemistry: 5-20µg/mL;1:35-140
Immunocytochemistry: 5-20µg/mL;1:35-140
Optimal working dilutions must be determined by end user.
Product No.
PAE640Hu01
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPE640Hu01-Recombinant Receptor Interacting Serine Threonine Kinase 1 (RIPK1)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
0.7mg/ml
Organism Species More
Mus musculus (Mouse)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against RIPK1. It has been selected for its ability to recognize RIPK1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Atherosclerosis | Vascular peroxide 1 promotes ox-LDL-induced programmed necrosis in endothelial cells through a mechanism involving β-catenin signaling Pubmed:29772481 |
| Cell and Tissue Research | Isolation and characterization of cluster of differentiation 9-positive ependymal cells as potential adult neural stem/progenitor cells in the third ventricle of adult rats Pubmed: 31788760 |
| Am J Respir Cell Mol Biol | Persistently Elevated Plasma Levels of RIPK3, MLKL, HMGB1, and RIPK1 in COVID-19 ICU Patients Pubmed:35385375 |
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