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SKU:PAF183Hu01

Polyclonal Antibody to 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2)

Polyclonal Antibody to 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

PAF183Hu01

Organism Species

Homo sapiens (Human).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPF183Hu01-Recombinant 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against HSD11b2. It has been selected for its ability to recognize HSD11b2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Journal of Allergy and Clinical Immunology Prenatal maternal distress affects atopic dermatitis in offspring mediated by oxidative stress Pubmed:27016803
Bioorganic Chemistry A novel derivatives of thiazol-4 (5H)-one and their activity in the inhibition of 11β-hydroxysteroid dehydrogenase type 1 Pubmed:29738970
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPF183Hu01 Recombinant 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) Positive Control; Immunogen; SDS-PAGE; WB.
PAF183Hu01 Polyclonal Antibody to 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) WB; IHC; ICC; IP.
MAF183Hu21 Monoclonal Antibody to 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) WB; IHC; ICC; IP.
SEF183Hu ELISA Kit for 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMF183Hu Magnetic Luminex Assay Kit for 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) ,etc. Magnetic Luminex Assay for Antigen Detection.
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