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Cloud-clone

SKU:PAF295Mu01

Polyclonal Antibody to Claudin 5 (CLDN5)

Polyclonal Antibody to Claudin 5 (CLDN5)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

PAF295Mu01

Organism Species

Mus musculus (Mouse).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

CPF295Mu21-OVA Conjugated Claudin 5 (CLDN5)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CLDN5. It has been selected for its ability to recognize CLDN5 in immunohistochemical staining and western blotting.

USAGE

Western blottin:g: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
ALLERGY ASTHMA & IMMUNOLOGY RESEARCH The Impact of the Endothelial Tight Junction Protein, Claudin-5, on the Clinical Profiles of Patients With COPD Pubmed:30088372
Int J Psychiatry Clin Pract Serum zonulin and claudin-5 levels in children with attention-deficit/hyperactivity disorder Pubmed: 32757874
Eur Arch Psychiatry Clin Neurosci Serum zonulin and claudin-5 levels in patients with schizophrenia Pubmed: 32564127
BIPOLAR DISORDERS Regulatory effect of lithium on hippocampal blood‐brain barrier integrity in a rat model of depressive‐like behavior Pubmed: 32558151
J Paediatr Child Health Zonulin and claudin‐5 levels in multisystem inflammatory syndrome and SARS‐CoV‐2 infection in children Pubmed:35638118
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPF295Mu03 Recombinant Claudin 5 (CLDN5) Positive Control; Immunogen; SDS-PAGE; WB.
RPF295Mu01 Recombinant Claudin 5 (CLDN5) Positive Control; Immunogen; SDS-PAGE; WB.
RPF295Mu02 Recombinant Claudin 5 (CLDN5) Positive Control; Immunogen; SDS-PAGE; WB.
CPF295Mu21 OVA Conjugated Claudin 5 (CLDN5) Immunogen; SDS-PAGE; WB.
PAF295Mu01 Polyclonal Antibody to Claudin 5 (CLDN5) WB; IHC; ICC; IP.
PAF295Mu02 Polyclonal Antibody to Claudin 5 (CLDN5) WB; IHC; ICC; IP.
SEF295Mu ELISA Kit for Claudin 5 (CLDN5) Enzyme-linked immunosorbent assay for Antigen Detection.
LMF295Mu Magnetic Luminex Assay Kit for Claudin 5 (CLDN5) ,etc. Magnetic Luminex Assay for Antigen Detection.
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