Cloud-clone
SKU:PAF822Mu01
Polyclonal Antibody to Phosphatase And Tensin Homolog (PTEN)
Polyclonal Antibody to Phosphatase And Tensin Homolog (PTEN)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAF822Mu01
Organism Species
Mus musculus (Mouse).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPF822Mu01-Recombinant Phosphatase And Tensin Homolog (PTEN)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against PTEN. It has been selected for its ability to recognize PTEN in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Experimental and Therapeutic Medicine | Elucidating the mechanism of miRNA‑214 in the regulation of gingival carcinoma 10.3892/etm.2017.4264 |
| Cancers (Basel) | In Vivo and In Vitro Enhanced Tumoricidal Effects of Metformin, Active Vitamin D3, and 5-Fluorouracil Triple Therapy against Colon Cancer by Modulating the … Pubmed:35326689 |
| Metformin and Calcitriol Enhance 5-Fluorouracil Tumoricidal Effects in Colon Cancer By Modulating The PI3K/Akt/PTEN/mTOR Network |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPF822Mu02 | Recombinant Phosphatase And Tensin Homolog (PTEN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPF822Mu01 | Recombinant Phosphatase And Tensin Homolog (PTEN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAF822Mu01 | Polyclonal Antibody to Phosphatase And Tensin Homolog (PTEN) | WB; IHC; ICC; IP. |
| PAF822Mu02 | Polyclonal Antibody to Phosphatase And Tensin Homolog (PTEN) | WB; IHC; ICC; IP. |
| SEF822Mu | ELISA Kit for Phosphatase And Tensin Homolog (PTEN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMF822Mu | Magnetic Luminex Assay Kit for Phosphatase And Tensin Homolog (PTEN) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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