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SKU:PAF878Mu02

Polyclonal Antibody to Secreted Frizzled Related Protein 4 (SFRP4)

Polyclonal Antibody to Secreted Frizzled Related Protein 4 (SFRP4)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

PAF878Mu02

Organism Species

Mus musculus (Mouse).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against SFRP4. It has been selected for its ability to recognize SFRP4 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Endocrine Journal Relationship between serum secreted frizzled-related protein 4 levels and the first-phase of glucose-stimulated insulin secretion in individuals with different glucose tolerance PubMed: 26041556
Horm Metab Res. Secreted Frizzled-Related Protein 4 (SFRP4) is Elevated in Patients with Diabetes Mellitus Pubmed:26882051
Obesity (Silver Spring).  Reduced expression of chemerin in visceral adipose tissue associates with hepatic steatosis in patients with obesity pubmed:27768254
Obesity Longitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican Americans Pubmed:29427376
Novel marker for gestational diabetes patent:US20180059122A1
British Journal Of Nutrition A diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profile Pubmed: 30375290
American Journal of Cancer Research SFRP4 is a prognostic marker and correlated with Treg cell infiltration in pancreatic ductal adenocarcinoma Pubmed: 30906634
Diabetologia Effects of long-term exercise on plasma adipokine levels and inflammation-related gene expression in subcutaneous adipose tissue in sedentary dysglycaemic … Pubmed: 31011777
PLoS One First trimester secreted Frizzled-Related Protein 4 and other adipokine serum concentrations in women developing gestational diabetes mellitus Pubmed: 33206702
Gastric Cancer SFRP4 drives invasion in gastric cancer and is an early predictor of recurrence 33277667
Diabetology Early Pregnancy Serum Concentration of Secreted Frizzled-Related Protein 4, Secreted Frizzled-Related Protein 5, and Chemerin in Obese Women Who Develop …
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPF878Mu02 Recombinant Secreted Frizzled Related Protein 4 (SFRP4) Positive Control; Immunogen; SDS-PAGE; WB.
RPF878Mu01 Recombinant Secreted Frizzled Related Protein 4 (SFRP4) Positive Control; Immunogen; SDS-PAGE; WB.
PAF878Mu01 Polyclonal Antibody to Secreted Frizzled Related Protein 4 (SFRP4) WB; IHC; ICC; IP.
PAF878Mu02 Polyclonal Antibody to Secreted Frizzled Related Protein 4 (SFRP4) WB; IHC; ICC; IP.
SEF878Mu ELISA Kit for Secreted Frizzled Related Protein 4 (SFRP4) Enzyme-linked immunosorbent assay for Antigen Detection.
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