Cloud-clone
SKU:MAA350Ra21
Monoclonal Antibody to Collagen Type I Alpha 1 (COL1a1)
Monoclonal Antibody to Collagen Type I Alpha 1 (COL1a1)
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Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Product No.
MAA350Ra21
Organism Species
Rattus norvegicus (Rat).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
C3
Ig Isotype
IgG1 Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPA350Ra01-Recombinant Collagen Type I Alpha 1 (COL1a1)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against COL1a1. It has been selected for its ability to recognize COL1a1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology | Simvastatin-loaded porous implant surfaces stimulate preosteoblasts differentiation: an in vitro study ScienceDirect: S1079210410004919 |
| Journal of Biomedical Materials | Osteoblast response to puerarin-loaded porous titanium surfaces: An in vitro study Wiley: source |
| European Archives of Oto-Rhino-Laryngology | Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats Pubmed: 24077847 |
| J Voice. | Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study Pubmed: 24495429 |
| Arch Oral Biol. | Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells. Pubmed: 24370188 |
| Laryngoscope. | Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria Pubmed:25545625 |
| UNIVERSA MEDICINA | Allogeneic human dermal fibroblasts are viable in peripheral blood mononuclear co-culture Univmed:Source |
| International Journal of Neuropsychopharmacology | The vitamin D receptor agonist, calcipotriol, modulates fibrogenic pathways mitigating liver fibrosis in-vivo: An experimental study. pubmed:27477355 |
| National Natural Scientific | Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure pubmed:29121396 |
| Nephron. | Prevention of Diabetic Nephropathy by Modified Acidic Fibroblast Growth Factor pubmed:28768285 |
| Cell and Tissue Research | Effect of TGFβ1, TGFβ3 and keratinocyte conditioned media on functional characteristics of dermal fibroblasts derived from reparative (Balb/c) and … Pubmed:29637306 |
| International Journal of Molecular Medicine | LOXL2, a copper-dependent monoamine oxidase, activates lung fibroblasts through the TGF-β/Smad pathway Pubmed: 30320382 |
| International Journal of Molecular Sciences | Association between Plasma HMGB-1 and Silicosis: A Case-Control Study Pubmed: 30558126 |
| Journal of Proteomics | Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection Pubmed: 31706945 |
| Brazilian Journal of Pharmaceutical Sciences | Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm |
| Ceramics International | Platinum nanoparticles supported on functionalized hydroxyapatite: Anti-oxidant properties and bone cells response |
| Scientific reports | Inhibition of eEF2K synergizes with glutaminase inhibitors or 4EBP1 depletion to suppress growth of triple-negative breast cancer cells 33911160 |
| Int J Mol Sci | Antiosteoporotic Nanohydroxyapatite Zoledronate Scaffold Seeded with Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration: A 3D In Vitro Model Pubmed:35682677 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA350Ra01 | Recombinant Collagen Type I Alpha 1 (COL1a1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA350Ra01 | Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1) | WB; IHC; ICC; IP. |
| MAA350Ra21 | Monoclonal Antibody to Collagen Type I Alpha 1 (COL1a1) | WB; IHC; ICC; IP. |
| SEA350Ra | ELISA Kit for Collagen Type I Alpha 1 (COL1a1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA350Ra | Magnetic Luminex Assay Kit for Collagen Type I Alpha 1 (COL1a1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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