Cloud-clone
SKU:PAJ103Mu02
Polyclonal Antibody to Filaggrin (FLG)
Polyclonal Antibody to Filaggrin (FLG)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAJ103Mu02
Organism Species
Mus musculus (Mouse).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPJ103Mu02-Recombinant Filaggrin (FLG)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against FLG. It has been selected for its ability to recognize FLG in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Journal of the Egyptian Women's Dermatologic Society | Filaggrin R501x and 2282del4 gene mutations and its tissue levels in patients with chronic plaque psoriasis LWW: 01000 |
| ?Inflammation & Allergy-Drug Targets | Neuromodulatory and Anti-Inflammatory Ingredient for Sensitive Skin: In Vitro Assessment Pubmed:24931812 |
| PLoS One | Protective Effects of Triphala on Dermal Fibroblasts and Human Keratinocytes Pubmed:26731545 |
| Journal of food biochemistry | In vitro anti-inflammatory and skin protective properties of extract comprising ceramides from de-oiled rice bran doi:10.1111 |
| European Journal of Pharmacology | Imiquimod-induced psoriasis-like inflammation in differentiated Human keratinocytes: Its evaluation using curcumin. pubmed:28736282 |
| Journal of Dermatological Science | A novel, topical, nonsteroidal, TRPV1 antagonist, PAC-14028 cream improves skin barrier function and exerts anti-inflammatory action through modulating epidermal … Pubmed:29752146 |
| Journal of Traditional and Complementary Medicine | In vitro anti-inflammatory and skin protective properties of Virgin coconut oil 10.1016:j.jtcme.2017.06.012 |
| Clinical and Molecular Allergy | Markers of atopic dermatitis, allergic rhinitis and bronchial asthma in pediatric patients: correlation with filaggrin, eosinophil major basic protein and … |
| Intractable & Rare Diseases Research | Filaggrin, major basic protein and leukotriene B4: Biomarkers for adult patients of bronchial asthma, atopic dermatitis and allergic rhinitis Pubmed: 30560019 |
| Dermatology and Therapy | Epidermal and Dermal Hallmarks of Photoaging are Prevented by Treatment with Night Serum Containing Melatonin, Bakuchiol, and Ascorbyl … |
| 日本化粧品技術者会誌 | 糖化に起因する表皮機能低下に対するワイルドタイムエキスの防御効果 |
| J Dermatol | Filaggrin‐gene mutation has minimal effect on the disease severity in the lesions of atopic dermatitis 34322929 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPJ103Mu03 | Recombinant Filaggrin (FLG) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPJ103Mu04 | Recombinant Filaggrin (FLG) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPJ103Mu01 | Recombinant Filaggrin (FLG) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPJ103Mu02 | Recombinant Filaggrin (FLG) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAJ103Mu01 | Polyclonal Antibody to Filaggrin (FLG) | WB; IHC; ICC; IP. |
| PAJ103Mu02 | Polyclonal Antibody to Filaggrin (FLG) | WB; IHC; ICC; IP. |
| LAJ103Mu81 | FITC-Linked Polyclonal Antibody to Filaggrin (FLG) | WB; IHC; ICC; IF. |
| LAJ103Mu71 | Biotin-Linked Polyclonal Antibody to Filaggrin (FLG) | WB; IHC; ICC. |
| SEJ103Mu | ELISA Kit for Filaggrin (FLG) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMJ103Mu | Magnetic Luminex Assay Kit for Filaggrin (FLG) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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