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SKU:MAA822Ra21

Monoclonal Antibody to Heat Shock Protein 60 (Hsp60)

Monoclonal Antibody to Heat Shock Protein 60 (Hsp60)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Product No.

MAA822Ra21

Organism Species

Rattus norvegicus (Rat).

Source

Monoclonal antibody preparation

Host

Mouse

Potency (Clone Number)

n/a

Ig Isotype

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

1mg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a mouse monoclonal antibody raised against Hsp60. It has been selected for its ability to recognize Hsp60 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Sci Rep Hsp60 response in experimental and human temporal lobe epilepsy PubMed: 25801186
Cellular and Molecular Gastroenterology and Hepatology Senescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitro Pubmed: 30878663
Breast Cancer Res Treat Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancer Pubmed: 32601970
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