Cloud-clone
SKU:MAA937Ra21
Monoclonal Antibody to Nephrin (NPHN)
Monoclonal Antibody to Nephrin (NPHN)
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Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Product No.
MAA937Ra21
Organism Species
Rattus norvegicus (Rat).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
C2
Ig Isotype
IgG2b Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPA937Ra01-Recombinant Nephrin (NPHN)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against NPHN. It has been selected for its ability to recognize NPHN in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Yonsei medical journal | The Level of Serum and Urinary Nephrin in Normal Pregnancy and Pregnancy with Subsequent Preeclampsia. pubmed:28120572 |
| Experimental and molecular pathology | Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. pubmed:29246788 |
| British Journal of Haematology | Renal protection by atorvastatin in a murine model of sickle cell nephropathy Pubmed:29527679 |
| Journal of Clinical Medicine | Multiplex Bead Array Assay of a Panel of Circulating Cytokines and Growth Factors in Patients with Albuminuric and Non-AlbuminuricDiabetic Kidney Disease Pubmed: 32961903 |
| Disease Markers | Urinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study 34707724 |
| Biomed Rep | Early biomarkers of nephrotoxicity associated with the use of anti-VEGF drugs Pubmed:35620307 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA937Ra01 | Recombinant Nephrin (NPHN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA937Ra01 | Polyclonal Antibody to Nephrin (NPHN) | WB; IHC; ICC; IP. |
| MAA937Ra21 | Monoclonal Antibody to Nephrin (NPHN) | WB; IHC; ICC; IP. |
| SEA937Ra | ELISA Kit for Nephrin (NPHN) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA937Ra | Magnetic Luminex Assay Kit for Nephrin (NPHN) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSA937Ra01 | ELISA Kit DIY Materials for Nephrin (NPHN) | Main materials for "Do It (ELISA Kit) Yourself". |
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